Increased expression of polypyrimidine tract binding protein results in higher insulin mRNA levels
2005 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 328, no 1, 38-42 p.Article in journal (Refereed) Published
The aim of this study was to further elucidate the role of the polypyrimidine tract binding protein (PTB) in the control of insulin mRNA stability. We observed that the glucose- or interleukin-1β-induced increase in insulin mRNA was paralleled by an increase in PTB mRNA. To further test the hypothesis that PTB controls insulin gene expression, βTC-6 cells were treated with a PTB-specific siRNA to modify the β-cell content of PTB. Surprisingly, we observed an increase in PTB mRNA and PTB protein levels in response to the siRNA treatment. In addition, the PTB-siRNA treatment also increased insulin mRNA. We conclude that expression of the PTB gene controls insulin production.
Place, publisher, year, edition, pages
2005. Vol. 328, no 1, 38-42 p.
Insulin mRNA, Polypyrimidine tract binding protein, mRNA stability, siRNA, β-cell
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-79787DOI: 10.1016/j.bbrc.2004.12.147PubMedID: 15670747OAI: oai:DiVA.org:uu-79787DiVA: diva2:107700