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Rate of translation of natural mRNAs in an optimized in vitro system
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology.
1996 (English)In: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, ISSN 0003-9861, Vol. 328, no 1, 9-16 p.Article in journal (Other scientific) Published
Abstract [en]

We report results on in vitro translation of an mRNA coding for elongation factor TuB which was in vitro transcribed from the tufB gene from Escherichia coil. Translation occurs at a rate of about 10 codons per second, which is close to the in vivo rate.

Place, publisher, year, edition, pages
ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS , 1996. Vol. 328, no 1, 9-16 p.
Keyword [en]
ribosomal mutants; elongation factors; in vitro translation; translation rate; natural mRNA; ESCHERICHIA-COLI RIBOSOMES; ELONGATION; GROWTH; INITIATION; INVITRO; GENE
Identifiers
URN: urn:nbn:se:uu:diva-80230OAI: oai:DiVA.org:uu-80230DiVA: diva2:108144
Note
Addresses: Pavlov MY, BIOMED CTR, DEPT BIOL MOLEC, BOX 590, S-75124 UPPSALA, SWEDEN.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-15

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