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5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2005 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 19, no 7, 773-779 p.Article in journal (Refereed) Published
Abstract [en]

5'-AMP-activated protein kinase (AMPK) activity is increased during exercise in an intensity- and glycogen-dependent manner. We previously reported that a mutation in the AMPK3 subunit (Prkag3225Q) increases AMPK activity and skeletal muscle glycogen content. Transfection experiments revealed the R225Q mutation is associated with high basal AMPK activity and diminished AMP dependence. Thus, the R225Q mutation can be considered a loss-of-function mutation that abolished allosteric regulation by AMP/ATP, causing increased basal AMPK activity. We used AMPK3 transgenic (Tg-Prkag3225Q) and knockout (Prkag3-/-) mice to determine the relationship between AMPK activity, glycogen content, and ergogenics (ability to perform work) in isolated extensor digitorum longus skeletal muscle after contractions induced by electrical stimulation. Contraction-induced AMPK activity was inversely coupled to glycogen content in wild-type and Tg-Prkag3225Q mice, but not in Prkag3-/- mice, highlighting a partial feedback control of glycogen on contraction-induced AMPK activity in the presence of a functional AMPK3 isoform. Skeletal muscle glycogen content was positively correlated to work performance, regardless of genotype. Thus, chronic activation of AMPK by the Prkag3225Q mutation directly influences skeletal muscle ergogenics by enhancing glycogen content. In conclusion, functional studies of the AMPK3 isoform further support the close connection between glycogen content and exercise performance in skeletal muscle.

Place, publisher, year, edition, pages
2005. Vol. 19, no 7, 773-779 p.
Keyword [en]
muscle ergogenics, AALPK activity, allosteric regulation, FDL, glycogen supercompensation
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-80242DOI: 10.1096/fj.04-3221comPubMedID: 15857891OAI: oai:DiVA.org:uu-80242DiVA: diva2:108156
Available from: 2006-05-04 Created: 2006-05-04 Last updated: 2017-12-14Bibliographically approved

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Andersson, Leif

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