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The kinetics of PDZ domain-ligand interactions and implications for the binding mechanism
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2005 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 280, no 41, 34805-34812 p.Article in journal (Refereed) Published
Abstract [en]

PDZ domains are protein adapter modules present in a few hundred human proteins. They play important roles in scaffolding and signal transduction. PDZ domains usually bind to the C termini of their target proteins. To assess the binding mechanism of this interaction we have performed the first in-solution kinetic study for PDZ domains and peptides corresponding to target ligands. Both PDZ3 from postsynaptic density protein 95 and PDZ2 from protein tyrosine phosphatase L1 bind their respective target peptides through an apparent A + B --> A.B mechanism without rate-limiting conformational changes. But a mutant with a fluorescent probe (Trp) outside of the binding pocket suggests that slight changes in the structure take place upon binding in protein tyrosine phosphatase-L1 PDZ2. For PDZ3 from postsynaptic density protein 95 the pH dependence of the binding reaction is consistent with a one-step mechanism with one titratable group. The salt dependence of the interaction shows that the formation of electrostatic interactions is rate-limiting for the association reaction but not for dissociation of the complex.

Place, publisher, year, edition, pages
2005. Vol. 280, no 41, 34805-34812 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-80270DOI: 10.1074/jbc.M506017200PubMedID: 16049001OAI: oai:DiVA.org:uu-80270DiVA: diva2:108184
Available from: 2006-05-05 Created: 2006-05-05 Last updated: 2017-10-16Bibliographically approved

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Engström, ÅkeJemth, Per

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Engström, ÅkeLarsson, MårtenChi N, CelestineJemth, Per
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