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Identification of I-ricinus, I-persulcatus and I-trianguliceps species by multiplex PCR
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Natl Inst Hlth Dev, Dept Virol, Tallinn, Estonia.;Tallinn Univ Technol, Dept Gene Technol, Tallinn, Estonia..
Natl Inst Hlth Dev, Dept Virol, Tallinn, Estonia..
Natl Inst Hlth Dev, Dept Virol, Tallinn, Estonia..
Univ Tartu, Dept Zool, Tartu, Estonia..
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2017 (English)In: Ticks and Tick-borne Diseases, ISSN 1877-959X, E-ISSN 1877-9603, Vol. 8, no 2, 235-240 p.Article in journal (Refereed) Published
Abstract [en]

Correct identification of tick species is an essential requirement for any scientific study engaged in tick associated research. However, morphological identification can lead to misinterpretations, especially when dealing with vector-host research and sub-adult, engorged or damaged specimens. To overcome this "limitation, we developed a novel assay to discriminate between Ixodes ricinus, I. persulcatus and I. trianguliceps species collected from rodents or vegetation, using the second internal transcribed spacer (ITS2) as a genetic marker. This single tube multiplex PCR allows specific amplification of targeted species and produces rapid and accurate results. The specificity was confirmed by sequencing the ITS2 and partial 16S rRNA genes from ticks collected from Estonia, Latvia, Sweden and Russia. We tested the assay in a large-scale experiment, and a total of 1284 ticks removed from rodents and shrews were successfully identified at species level.

Place, publisher, year, edition, pages
2017. Vol. 8, no 2, 235-240 p.
Keyword [en]
I. ricinus, I. persulcatus, I. trianguliceps, ITS2, Species identification
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-317701DOI: 10.1016/j.ttbdis.2016.11.004ISI: 000393256700005PubMedID: 27856176OAI: oai:DiVA.org:uu-317701DiVA: diva2:1082652
Available from: 2017-03-17 Created: 2017-03-17 Last updated: 2017-03-17Bibliographically approved

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Värv, KairiLundkvist, Åke
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