Identification of I-ricinus, I-persulcatus and I-trianguliceps species by multiplex PCR
2017 (English)In: Ticks and Tick-borne Diseases, ISSN 1877-959X, E-ISSN 1877-9603, Vol. 8, no 2, 235-240 p.Article in journal (Refereed) Published
Correct identification of tick species is an essential requirement for any scientific study engaged in tick associated research. However, morphological identification can lead to misinterpretations, especially when dealing with vector-host research and sub-adult, engorged or damaged specimens. To overcome this "limitation, we developed a novel assay to discriminate between Ixodes ricinus, I. persulcatus and I. trianguliceps species collected from rodents or vegetation, using the second internal transcribed spacer (ITS2) as a genetic marker. This single tube multiplex PCR allows specific amplification of targeted species and produces rapid and accurate results. The specificity was confirmed by sequencing the ITS2 and partial 16S rRNA genes from ticks collected from Estonia, Latvia, Sweden and Russia. We tested the assay in a large-scale experiment, and a total of 1284 ticks removed from rodents and shrews were successfully identified at species level.
Place, publisher, year, edition, pages
2017. Vol. 8, no 2, 235-240 p.
I. ricinus, I. persulcatus, I. trianguliceps, ITS2, Species identification
Infectious Medicine Microbiology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-317701DOI: 10.1016/j.ttbdis.2016.11.004ISI: 000393256700005PubMedID: 27856176OAI: oai:DiVA.org:uu-317701DiVA: diva2:1082652