A human protein atlas for normal and cancer tissues based on antibody proteomics
2005 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 4, no 12, 1920-1932 p.Article in journal (Refereed) Published
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, ∼400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
Place, publisher, year, edition, pages
2005. Vol. 4, no 12, 1920-1932 p.
Antibodies/*chemistry/isolation & purification, Antibodies; Neoplasm/*chemistry/isolation & purification, Blotting; Western, Chromatography; Affinity, Databases; Protein, Epitopes/chemistry, Expressed Sequence Tags, Humans, Neoplasms/genetics/*immunology, Proteins/immunology, Proteome/*immunology/isolation & purification, Reference Values, Research Support; Non-U.S. Gov't
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-80605DOI: 10.1074/mcp.M500279-MCP200PubMedID: 16127175OAI: oai:DiVA.org:uu-80605DiVA: diva2:108519