Urine profiling using capillary electrophoresis-mass spectrometry and multivariate data analysis
2006 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1, no 1117, 87-93 p.Article in journal (Refereed) Published
This work presents the development of a general and fast method for metabolic profiling of urine, using capillary electrophoresis-electrospray ionisation mass spectrometry (CE-ESIMS) and multivariate data analysis (DA). Human urine samples collected before and after ingestion of paracetamol were analysed at acidic and basic CE conditions, using both positive and negative ESI-MS detection. Analysis of the entire resulting data set, with no prior knowledge of the target compounds, using pair-wise 'fuzzy' correlation and eigenvalue analysis enabled the samples to be discriminated between on the basis of blank urine and urine collected after drug intake. By generating two-dimensional loadings plots, it was also possible to identify the m/z values of the substances responsible for the differentiation between control and dosed samples.
Place, publisher, year, edition, pages
2006. Vol. 1, no 1117, 87-93 p.
metabonomics, electrospray ionisation mass spectrometry, capillary electrophoresis, urine, biomarkers, metabolite, paracetamol, multivariate data analysis, fuzzy correlation
IdentifiersURN: urn:nbn:se:uu:diva-80651DOI: doi:10.1016/j.chroma.2006.03.048PubMedID: 16620839OAI: oai:DiVA.org:uu-80651DiVA: diva2:108565