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Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β
Inserm U1052, Centre de Recherche en Cancérologie de Lyon, 69000, Lyon, France.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research. Uppsala University, Science for Life Laboratory, SciLifeLab. (Aristidis Moustakas)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research. Uppsala University, Science for Life Laboratory, SciLifeLab. Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan..
Inserm U1052, Centre de Recherche en Cancérologie de Lyon, 69000, Lyon, France.
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2016 (English)In: TGF-Beta Signaling: Methods and Protocols / [ed] Feng, XH ; Xu, P ; Lin, X, Springer, 2016, Vol. 1344, 147-181 p.Chapter in book (Refereed)
Abstract [en]

In recent years, the importance of the cell biological process of epithelial-mesenchymal transition (EMT) has been established via an exponentially growing number of reports. EMT has been documented during embryonic development, tissue fibrosis, and cancer progression in vitro, in animal models in vivo and in human specimens. EMT relates to many molecular and cellular alterations that occur when epithelial cells undergo a switch in differentiation that generates mesenchymal-like cells with newly acquired migratory and invasive properties. In addition, EMT relates to a nuclear reprogramming similar to the one occurring in the generation of induced pluripotent stem cells. Via such a process, EMT is gradually established to promote the generation and maintenance of adult tissue stem cells which under disease states such as cancer, are known as cancer stem cells. EMT is induced by developmental growth factors, oncogenes, radiation, and hypoxia. A prominent growth factor that causes EMT is transforming growth factor β (TGF-β).A series of molecular and cellular techniques can be applied to define and characterize the state of EMT in diverse biological samples. These methods range from DNA and RNA-based techniques that measure the expression of key EMT regulators and markers of epithelial or mesenchymal differentiation to functional assays of cell mobility, invasiveness and in vitro stemness. This chapter focuses on EMT induced by TGF-β and provides authoritative protocols and relevant reagents and citations of key publications aiming at assisting newcomers that enter this prolific area of biomedical sciences, and offering a useful reference tool to pioneers and aficionados of the field.

Place, publisher, year, edition, pages
Springer, 2016. Vol. 1344, 147-181 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1344
Keyword [en]
Cellular plasticity, Epithelial–mesenchymal transition, Invasion, Motility, Signal transduction, Transcription factor, Transforming growth factor β
National Category
Cell and Molecular Biology
Research subject
Medical Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-319069DOI: 10.1007/978-1-4939-2966-5_9PubMedID: 26520123OAI: oai:DiVA.org:uu-319069DiVA: diva2:1086101
Available from: 2017-03-31 Created: 2017-03-31 Last updated: 2017-03-31Bibliographically approved

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