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Somatic Ephrin Receptor Mutations Are Associated with Metastasis in Primary Colorectal Cancer
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Experimental and Clinical Oncology.
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2017 (English)In: Cancer Research, ISSN 0008-5472, E-ISSN 1538-7445, Vol. 7, no 77, 1730-1740 p.Article in journal (Refereed) Published
Abstract [en]

The contribution of somatic mutations to metastasis of colorectal cancers is currently unknown. To find mutations involved in the colorectal cancer metastatic process, we performed deep mutational analysis of 676 genes in 107 stages II to IV primary colorectal cancer, of which half had metastasized. The mutation prevalence in the ephrin (EPH) family of tyrosine kinase receptors was 10-fold higher in primary tumors of metastatic colorectal than in nonmetastatic cases and preferentially occurred in stage III and IV tumors. Mutational analyses in situ confirmed expression of mutant EPH receptors. To enable functional studies of EPHB1 mutations, we demonstrated that DLD-1 colorectal cancer cells expressing EPHB1 form aggregates upon coculture with ephrin B1 expressing cells. When mutations in the fibronectin type III and kinase domains of EPHB1 were compared with wild-type EPHB1 in DLD-1 colorectal cancer cells, they decreased ephrin B1-induced compartmentalization. These observations provide a mechanistic link between EPHB receptor mutations and metastasis in colorectal cancer.

Place, publisher, year, edition, pages
2017. Vol. 7, no 77, 1730-1740 p.
National Category
Clinical Laboratory Medicine Cancer and Oncology
Research subject
Pathology
Identifiers
URN: urn:nbn:se:uu:diva-319146DOI: 10.1158/0008-5472.CAN-16-1921ISI: 000398262400019PubMedID: 28108514OAI: oai:DiVA.org:uu-319146DiVA: diva2:1086168
Funder
Swedish Cancer Society, 2006/2154; 2007/775; 2012/834EU, European Research Council, 601939Swedish Foundation for Strategic Research , F06-0050VINNOVA
Available from: 2017-03-31 Created: 2017-03-31 Last updated: 2017-06-28

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Mathot, LucyKundu, SnehangshuLjungström, ViktorMoens, Lotte
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