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Microbial community assembly and evolution in subseafloor sediment
Aarhus Univ, Ctr Geomicrobiol, Dept Biosci, Microbiol Sect, DK-8000 Aarhus, Denmark..
Aarhus Univ, Bioinformat Res Ctr, DK-8000 Aarhus C, Denmark..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala University, Science for Life Laboratory, SciLifeLab.
Aarhus Univ, Ctr Geomicrobiol, Dept Biosci, Microbiol Sect, DK-8000 Aarhus, Denmark..
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2017 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 114, no 11, 2940-2945 p.Article in journal (Refereed) Published
Abstract [en]

Bacterial and archaeal communities inhabiting the subsurface seabed live under strong energy limitation and have growth rates that are orders of magnitude slower than laboratory-grown cultures. It is not understood how subsurface microbial communities are assembled and whether populations undergo adaptive evolution or accumulate mutations as a result of impaired DNA repair under such energy-limited conditions. Here we use amplicon sequencing to explore changes of microbial communities during burial and isolation from the surface to the >5,000-y-old subsurface of marine sediment and identify a small core set of mostly uncultured bacteria and archaea that is present throughout the sediment column. These persisting populations constitute a small fraction of the entire community at the surface but become predominant in the subsurface. We followed patterns of genome diversity with depth in four dominant lineages of the persisting populations by mapping metagenomic sequence reads onto single-cell genomes. Nucleotide sequence diversity was uniformly low and did not change with age and depth of the sediment. Likewise, there was no detectable change inmutation rates and efficacy of selection. Our results indicate that subsurface microbial communities predominantly assemble by selective survival of taxa able to persist under extreme energy limitation.

Place, publisher, year, edition, pages
2017. Vol. 114, no 11, 2940-2945 p.
Keyword [en]
marine sediment, bacteria, metagenomics, evolution, single-cell genomics
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-318919DOI: 10.1073/pnas.1614190114ISI: 000396094200052PubMedID: 28242677OAI: oai:DiVA.org:uu-318919DiVA: diva2:1087036
Funder
EU, FP7, Seventh Framework Programme, 294200EU, European Research CouncilSwedish Foundation for Strategic Research , SSF-FFL5
Available from: 2017-04-05 Created: 2017-04-05 Last updated: 2017-04-06Bibliographically approved

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Ettema, Thijs J. G.
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