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Differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by LC-MS/MS comparison of their skin peptidomes
Moscow MV Lomonosov State Univ, Dept Chem, Leninskie Gori 1-3, Moscow 119991, Russia..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Univ Ljubljana, Fac Hlth Sci, Zdravstvena Pot 5, Ljubljana 1000, Slovenia..
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2017 (English)In: Analusis, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 409, no 7, 1951-1961 p.Article in journal (Refereed) Published
Abstract [en]

LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus, it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus. The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family.

Place, publisher, year, edition, pages
SPRINGER HEIDELBERG , 2017. Vol. 409, no 7, 1951-1961 p.
Keyword [en]
Natural products, Frog peptides, Brevinins, Green frogs, Tandem mass spectrometry, Peptide sequencing
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-319540DOI: 10.1007/s00216-016-0143-3ISI: 000394377200023PubMedID: 28012108OAI: oai:DiVA.org:uu-319540DiVA: diva2:1087235
Available from: 2017-04-06 Created: 2017-04-06 Last updated: 2017-04-06Bibliographically approved

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