uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Human Allergen-Specific IgG Subclass Antibodies Measured Using ImmunoCAP Technology
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Respiratory Medicine and Allergology. Thermo Fisher Sci, ImmunoDiagnost, POB 6460, SE-75137 Uppsala, Sweden..
Thermo Fisher Sci, ImmunoDiagnost, POB 6460, SE-75137 Uppsala, Sweden..
Thermo Fisher Sci, ImmunoDiagnost, POB 6460, SE-75137 Uppsala, Sweden..
Unilever SEAC, Colworth Sci Pk, Sharnbrook, Beds, England..
Show others and affiliations
2017 (English)In: International Archives of Allergy and Immunology, ISSN 1018-2438, E-ISSN 1423-0097, Vol. 172, no 1, 1-10 p.Article in journal (Refereed) Published
Abstract [en]

Background: Knowledge of human IgG subclass antibody responses to various allergens has been hampered by a lack of reliable standardized assays. The aim here was to develop quantitative immunoassays for human IgG1, IgG2, and IgG3 antibodies using ImmunoCAP (R) technology and to evaluate their application. Methods: Enzyme conjugates with isotype-specific monoclonal antibodies and calibrators composed of purified myeloma paraproteins were developed for each assay and used together with other standardized assay reagents for the Phadia (R) 100 instrument. The calibrators were adjusted to the international reference preparation IRP 67/86. The assays were characterized and used together with other standard ImmunoCAP assays to measure antibodies to various allergens in preliminary studies. Results: The new assays had limits of quantitation of 1.0 (IgG1), 4.6 (IgG2), and 0.04 mg(A)/L (IgG3), and coefficients of variation of <20%. Only some minor cross -reactivity with IgG2 was observed for the specific IgG1 assay. The specific IgG2 assay showed a bias for the allotype G2m(23) and compensation factors were used to adjust the measured concentrations accordingly. Preliminary studies indicated a strong and stable IgG4 antibody response to P-lactoglobulin in healthy individuals, a high IgG1 and even higher IgG2 antibody response to house dust mite in sensitized and nonsensitized subjects, and a mixed IgG subclass response to venom allergens in allergic patients with increasing IgG4 antibody levels during venom immunotherapy. Conclusions: The new research assays are valuable tools for immunological studies, enabling the characterization of antibody profiles using a standardized approach, and facilitating data interpretation and the comparison of results across studies.

Place, publisher, year, edition, pages
KARGER , 2017. Vol. 172, no 1, 1-10 p.
Keyword [en]
Allergen, Antibody, IgG subclass, Immunoassay, InnmunoCAP
National Category
Respiratory Medicine and Allergy
Identifiers
URN: urn:nbn:se:uu:diva-319687DOI: 10.1159/000455098ISI: 000397364500001PubMedID: 28219072OAI: oai:DiVA.org:uu-319687DiVA: diva2:1087430
Available from: 2017-04-07 Created: 2017-04-07 Last updated: 2017-04-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Movérare, Robert
By organisation
Respiratory Medicine and Allergology
In the same journal
International Archives of Allergy and Immunology
Respiratory Medicine and Allergy

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 116 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf