Why mitochondria need a genome revisited
2017 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 591, no 1, 65-75 p.Article in journal (Refereed) Published
In this paper, we experimentally address the debate about why functional transfer of mitochondrial genes to the nucleus has been halted in some organismal groups and why cytosolic expression of mitochondrial proteins has proven remarkably difficult. By expressing all 13 human mitochondrial-encoded genes with strong mitochondrial-targeting sequences in the cytosol of human cells, we show that all proteins, except ATP8, are transported to the endoplasmic reticulum (ER). These results confirm and extend previous findings based on three mitochondrial genes lacking mitochondrial-targeting sequences that also were relocated to the ER during cytosolic expression. We conclude that subcellular protein targeting constitutes a major barrier to functional transfer of mitochondrial genes to the nuclear genome.
Place, publisher, year, edition, pages
WILEY-BLACKWELL , 2017. Vol. 591, no 1, 65-75 p.
endoplasmic reticulum, hydrophobicity, mitochondria, signal recognition particle, transmembrane domain
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-319810DOI: 10.1002/1873-3468.12510ISI: 000393957400008PubMedID: 27928819OAI: oai:DiVA.org:uu-319810DiVA: diva2:1087856
FunderSwedish Research Council, 349-2007-8732 621-2014-4460Knut and Alice Wallenberg Foundation, 2011.0148 2012.0075German Research Foundation (DFG)