Excitation- and emission wavelength-based multiplex imaging using red-absorbing near infrared-emitting lanthanide complexes
(English)Manuscript (preprint) (Other academic)
: Multiplex luminescence imaging in the red and near infrared (NIR) should be an enabling tool for the real-time investigation of complex biological systems. Current probes have numerous limitations including low photostability, short luminescent lifetime, overlapping absorption and emission bands, and small Stokes shift, the combination of which limits multiplexing in this region to two colors. Here we report emitters based on near infrared (NIR) emitting luminescent lanthanides (Ln) sensitized by hydroporphyin antennae. The narrow and intense absorption bands of hydroporphyrins are in the red, and their porisiotns are readily tunable. These dyads enable both emission- and excitation-based multiplexing; the former by combining either the same antenna with different Ln’s, the latter by attaching chlorin antennae with non-overlapping absorptions to the same Ln-complex. Up to 4 different species could be discerned without tedious data manipulation.
Research subject Chemistry with specialization in Organic Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-319907OAI: oai:DiVA.org:uu-319907DiVA: diva2:1087971