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Yeast Elf1 factor is phosphorylated and interacts with protein kinase CK2
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research.
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2006 (English)In: Journal of Biochemistry and Molecular Biology, ISSN 1225-8687, E-ISSN 0219-1024, Vol. 39, no 3, 311-318 p.Article in journal (Refereed) Published
Abstract [en]

One of the biggest group of proteins influenced by protein kinase CK2 is formed by factors engaged in gene expression. Here we have reported recently identified yeast transcription elongation factor Elf1 as a new substrate for both monomeric and tetrameric forms of CK2. Elf1 serves as a substrate for both the recombinant CK2alpha' (K(m) 0.38 microM) and holoenzyme (K(m) 0.13 microM). By MALDI-MS we identified the two serine residues at positions 95 and 117 as the most probable in vitro phosphorylation sites. Coimmunoprecypitation experiments show that Elf1 interacts with catalytic (alpha and alpha') as well as regulatory (beta and beta') subunits of CK2. These data may help to elucidate the role of protein kinase CK2 and Elf1 in the regulation of transcription elongation.

Place, publisher, year, edition, pages
2006. Vol. 39, no 3, 311-318 p.
Keyword [en]
Cloning and overexpression, Mass spectrometry, Phosphorylation, Protein-protein interaction, Protein kinase CK2, Transcription, Transcription factor Elf1, Yeast
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-80913PubMedID: 16756761OAI: oai:DiVA.org:uu-80913DiVA: diva2:108827
Available from: 2006-06-20 Created: 2006-06-20 Last updated: 2017-12-14Bibliographically approved

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Hellman, Ulf

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