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Expression of Transient Receptor Potential Channels in the Purified Human Pancreatic beta-Cells
Soder Sjukhuset, Ctr Mol Med, Res Ctr, Karolinska Inst,Unit Computat Med, Stockholm, Sweden.;Soder Sjukhuset, Res Ctr, Karolinska Inst, Bioinformat Infrastruct Life Sci, Stockholm, Sweden.;Soder Sjukhuset, Res Ctr, Karolinska Inst, Dept Clin Sci & Educ, 3rd floor, SE-11883 Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. Soder Sjukhuset, Res Ctr, Karolinska Inst, Dept Clin Sci & Educ, 3rd floor, SE-11883 Stockholm, Sweden..
2017 (English)In: Pancreas, ISSN 0885-3177, E-ISSN 1536-4828, Vol. 46, no 1, p. 97-101Article in journal (Refereed) Published
Abstract [en]

Objective: Members of the transient receptor potential (TRP) channels are involved in mediating the electrical excitability and stimulus-secretion coupling in the pancreatic beta-cells. The expression and the relative abundance of different TRP channels in the human beta-cells are unknown. The objective of this study was to examine the expression of the TRP channels and their relative abundance in the human beta-cell. Methods: RNA sequencing data obtained from human islets, fluorescence-activated cell sorting-purified human beta-cell and human pancreatic acinar cells were analyzed. Gene counts and fragments per kilobase per million mapped reads were obtained. Results: Among the TRPC family only the TRPC1 was expressed in the human beta-cell. TRPV1 channels were not expressed in the human beta-cells. Among the TRPM family, TRPM4, TRPM7, TRPM2, and TRPM3 were expressed in the human beta-cell. Of the remaining TRP channels, TRPP2, TRPML1, and TRPML3 were expressed in these cells. Conclusions: By analyzing the RNA sequencing data, we have detected for the first time the TRP channels that are expressed in the purified human beta-cells, in comparison to the other relevant pancreatic cell types. Our study provides an opportunity to focus on these TRP channels for a better understanding of the electrophysiology and stimulus-secretion coupling in these cells.

Place, publisher, year, edition, pages
LIPPINCOTT WILLIAMS & WILKINS , 2017. Vol. 46, no 1, p. 97-101
Keywords [en]
transient receptor potential channel, pancreatic beta-cells, RNA sequencing, calcium signaling, islets of Langerhans, signal transduction
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:uu:diva-320820DOI: 10.1097/MPA.0000000000000685ISI: 000397644300011PubMedID: 27464700OAI: oai:DiVA.org:uu-320820DiVA, id: diva2:1091128
Funder
The Karolinska Institutet's Research FoundationAvailable from: 2017-04-26 Created: 2017-04-26 Last updated: 2017-04-26Bibliographically approved

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