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Quantitative proteomic analysis of gastric cancer tissue reveals novel proteins in platelet-derived growth factor B signaling pathway
Binzhou Med Univ, Med & Pharm Res Ctr, Yantai 264003, Shandong, Peoples R China.;Binzhou Med Univ, Dept Radiol, Affiliated Hosp, Binzhou 256603, Shandong, Peoples R China..
Binzhou Med Univ, Med & Pharm Res Ctr, Yantai 264003, Shandong, Peoples R China..
Binzhou Med Univ, Med & Pharm Res Ctr, Yantai 264003, Shandong, Peoples R China..
Binzhou Med Univ, Dept Radiol, Affiliated Hosp, Binzhou 256603, Shandong, Peoples R China..
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2017 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 8, no 13, p. 22059-22075Article in journal (Refereed) Published
Abstract [en]

Gastric cancer is one of the most common cancers in Asian countries. Searching for reliable biomarkers involving the development of gastric cancer is important for clinical practice. Quantitative proteomics has become an important method contributed to the discovery of novel diagnostic or therapeutic targets for the management of cancer. Here, we identified differently expressed proteins in gastric cancer and normal gastric tissues by using the high resolution mass spectrometer. Among the total of 2280 identified proteins, 87 were differentially expressed between gastric cancer and normal gastric tissues. Notably, several significant proteins are in the PDGF-B signaling pathway, including peroxiredoxin5 (PRDX5), S100A6, calreticulin (CALR) and cathepsin D (CTSD), which were validated by western blot. Furthermore, upstream regulators including PDGF-B, PDGFR-beta, Akt, eIF4E and p70s6K were found significantly increased in the gastric cancer tissues. In addition, silencing of PRDX5 and PDGF-B suppressed the proliferation of gastric cancer cells in vitro. The administration of exogenous PDGF-BB recovered the reduced expression of PDGF-B signaling pathway in PDGF-B knockdown cells. Taken together, our findings suggested that PDGF-B signaling pathway plays an important role in the regulation of gastric cancer proliferation and the inhibition of this pathway may be a potential approach for treatment of gastric cancer.

Place, publisher, year, edition, pages
IMPACT JOURNALS LLC , 2017. Vol. 8, no 13, p. 22059-22075
Keywords [en]
proteomic, PDGF-B, gastric cancer, PRDX5, pathway
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:uu:diva-320818DOI: 10.18632/oncotarget.15908ISI: 000397642400127PubMedID: 28423550OAI: oai:DiVA.org:uu-320818DiVA, id: diva2:1091135
Funder
Swedish Research Council, 2015-4870Available from: 2017-04-26 Created: 2017-04-26 Last updated: 2017-11-29Bibliographically approved

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Bergquist, JonasMi, Jia

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