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Human papillomavirus DNA detection in fine-needle aspirates as indicator of human papillomavirus-positive oropharyngeal squamous cell carcinoma: A prospective study
Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden..
Karolinska Inst, CLINTEC, Dept Otorhinolaryngol Head & Neck Surg, Stockholm, Sweden..
Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden..
Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden..
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2017 (English)In: Head and Neck, ISSN 1043-3074, E-ISSN 1097-0347, Vol. 39, no 3, 419-426 p.Article in journal (Refereed) Published
Abstract [en]

Background. Human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (SCC) has a better outcome than most head neck squamous cell carcinomas (HNSCCs) and an HPV-positive lymph node metastasis likely has an HPV-positive oropharyngeal SCC origin. Determining HPV-status in cervical lymph nodes by fine-needle aspiration cytology (FNAC) may be useful for diagnosis. Methods. FNACs from 66 patients with neck masses were prospectively examined for HPV DNA and HPV16 mRNA by a polymerase chain reaction (PCR)-based assay, and the data correlated to diagnosis and HPV-status obtained from histopathological specimens. Results. Aspirates from 17 of 66 patients, later diagnosed with HPV-positive oropharyngeal SCC, were HPV16 DNA-positive. HPV16 mRNA was detected in all cases with extractable RNA. All remaining FNACs, including 18 branchial cleft cysts, were HPV DNA-negative. HPV DNA status in the aspirates showed perfect concordance with corresponding biopsies. Conclusion. HPV16 DNA detection in fine-needle aspirations from neck masses is reliable and HPV16 DNA in a metastasis is a strong indicator of an HPV-positive oropharyngeal SCC.

Place, publisher, year, edition, pages
Wiley , 2017. Vol. 39, no 3, 419-426 p.
Keyword [en]
human papillomavirus, head and neck cancer, oropharyngeal cancer, fine-needle aspiration, fine-needle biopsy
National Category
Microbiology in the medical area Cancer and Oncology Otorhinolaryngology
Identifiers
URN: urn:nbn:se:uu:diva-320276DOI: 10.1002/hed.24641ISI: 000397334700006PubMedID: 27898186OAI: oai:DiVA.org:uu-320276DiVA: diva2:1092005
Funder
Swedish Cancer SocietyCancer and Allergy FoundationStockholm County Council
Available from: 2017-04-28 Created: 2017-04-28 Last updated: 2017-04-28Bibliographically approved

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Microbiology in the medical areaCancer and OncologyOtorhinolaryngology

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