Bacteria counting with impedance spectroscopy in a micro probe station
2006 (English)In: Journal of physical chemistry B, ISSN 1520-6106, Vol. 110, no 20, 10165-10169 p.Article in journal (Refereed) Published
A method to quantify the density of viable biological cells in suspensions is presented. The method is implemented by low-frequency impedance spectroscopy and based on the finding that immobilized ions are released to move freely in the surrounding suspension when viable Escherichia coli cells are killed by a heat shock. The presented results show that an amount of ions corresponding to 2 × 108 unit charges are released per viable bacterium killed. A micro probe station with coplanar Ti electrodes was electrically characterized and used as a measuring unit for the impedance spectroscopy recordings. This unit is compatible with common microfabrication techniques and should enable the presented method to be employed using a flow-cell device for viable bacteria counting in miniaturized on-line monitoring systems.
Place, publisher, year, edition, pages
2006. Vol. 110, no 20, 10165-10169 p.
Other Materials Engineering
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-81756DOI: 10.1021/jp060148qPubMedID: 16706478OAI: oai:DiVA.org:uu-81756DiVA: diva2:109671