uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Strong binding of bioactive BMP-2 to nanocrystalline diamond by physisorption
Show others and affiliations
2006 (English)In: Biomaterials, Vol. 27, 4547-4556 p.Article in journal (Refereed) Published
Abstract [en]

Nano-crystalline diamond (NCD)-coated surfaces were efficiently functionalized with bone morphogenetic protein-2 (BMP-2) by means of physisorption. Due to their randomly oriented texture, NCD-coated surfaces appear to bind complex molecules firmly. Applying various highly sensitive analytical methods, the interaction was found extremely stable. The strength of the experimentally measured adherence between BMP-2 and NCD was further corroborated by theoretical calculations. Oxygen treatment rendered NCD hydrophilic by the appearance of surface oxygen containing groups. This particular NCD surface exhibited even higher binding energies towards BMP-2 than the hydrophobic surface, and this surface was also favoured by cultured cells. Most importantly in this context, bound BMP-2 was found fully active. When cultured on BMP-2-treated NCD, osteosarcoma cells strongly up-regulated alkaline phosphatase, a specific marker for osteogenic differentiation. Hence, this simple method will allow generating highly versatile surfaces with complex biomimetic coatings, essentials for novel medical devices and implants as well as for innovative scaffolds in tissue engineering.

Place, publisher, year, edition, pages
2006. Vol. 27, 4547-4556 p.
Keyword [en]
Physisorption, BMP-2, Bone tissue engineering, Nanocrystalline, Diamond
National Category
Inorganic Chemistry
URN: urn:nbn:se:uu:diva-82100DOI: doi:10.1016/j.biomaterials.2006.04.036OAI: oai:DiVA.org:uu-82100DiVA: diva2:110015
Available from: 2006-09-18 Created: 2006-09-18 Last updated: 2011-01-11

Open Access in DiVA

No full text

Other links

Publisher's full texthttp://www.sciencedirect.com

Search in DiVA

By author/editor
Larsson, Karin
By organisation
Department of Materials ChemistryInorganic Chemistry
Inorganic Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 407 hits
ReferencesLink to record
Permanent link

Direct link