uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Properties Of Carrot Polyphenoloxidase
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
1995 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 39, no 1, 33-38 p.Article in journal (Refereed) Published
Abstract [en]

A latent phenoloxidase (PPO) was purified in the presence of protease inhibitors from carrot cells to electrophoretic homogeneity. The inactive enzyme had a M(r) of ca 59 000 under denaturating conditions as judged by SDS-PAGE. When stored in the absence of protease inhibitors, PPO in a crude extract was converted to forms with a lower M(r). Phenol oxidase activity of the purified PPO was induced by the presence in the assay medium of 12.5 mM Tris, and 6 mM CaCl2 increased the activity further.

Place, publisher, year, edition, pages
1995. Vol. 39, no 1, 33-38 p.
Keyword [en]
Daucus Carota, Umbelliferae, Carrot, Latent Enzyme, Phenoloxidase, Prophenoloxidase, Purification, Broad Bean Polyphenoloxidase, Glandular Trichomes, Cell-Cultures, Oxidase, Purification, Expression, Activation, Cleavage, Prophenoloxidase, Proteins
National Category
URN: urn:nbn:se:uu:diva-82824DOI: 10.1016/0031-9422(94)00918-JISI: A1995RB04800006OAI: oai:DiVA.org:uu-82824DiVA: diva2:110731
Available from: 2006-10-13 Created: 2006-10-13 Last updated: 2014-08-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Söderhäll, Irene
By organisation
Comparative Physiology
In the same journal

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 273 hits
ReferencesLink to record
Permanent link

Direct link