uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Uncoordinated Expression of Myosin Heavy Chains and Myosin-Binding Protein C Isoforms in Human Extraocular Muscles
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Show others and affiliations
2006 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, Vol. 47, no 10, 4188-4193 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE. To examine the distribution of myosin-binding protein C (MyBP-C) in human extraocular muscles (EOMs) and to correlate the myosin heavy chain (MyHC) and the MyBP-C composition of the fibers.

METHODS. Samples from 17 EOMs, 3 levator palpebrae ( LP), and 6 limb muscles were analyzed with SDS-PAGE and immunoblot or processed for immunocytochemistry with monoclonal antibodies (mAbs) against MyBP-C-fast, MyBP-C-slow, MyHCIIa, MyHCI, MyHCsto, MyHC alpha-cardiac, and MyHCemb.

RESULTS. In the limb muscle samples, fast fibers were labeled with anti-MyBP-C-fast and anti-MyBP-C-slow, whereas the slow fibers were immunostained with anti-MyBP-C-slow only, in accordance with previous studies. In 11 EOM samples MyBPC-fast was not detected, and weak staining with anti-MyBP-Cfast was seen only in a few fibers in the proximal part of 2 muscles. The mAb against MyBP-C-slow labeled all fibers, but fibers containing MyHCI were generally more strongly stained. In the levator palpebrae, immunostaining with anti-MyBP-C-fast was present in some fibers labeled with anti-MyHCIIa and/ or anti-MyHCeom. MyBP-C-fast and -intermediate were not detected biochemically in the EOMs.

CONCLUSIONS. The lack of MyBP-C-fast and intermediate is an additional feature of the human EOM allotype. The true EOMs have a unique myofibrillar protein isoform composition reflecting their special structural and functional properties. The levator palpebrae muscle phenotype is intermediate between that of the EOMs and the limb muscles.

Place, publisher, year, edition, pages
2006. Vol. 47, no 10, 4188-4193 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-83285DOI: 10.1167/iovs.05-1496ISI: 000240784700002PubMedID: 17003405OAI: oai:DiVA.org:uu-83285DiVA: diva2:111193
Available from: 2006-10-27 Created: 2006-10-27 Last updated: 2011-06-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Neuroscience
In the same journal
Investigative Ophthalmology and Visual Science
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 147 hits
ReferencesLink to record
Permanent link

Direct link