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A mouse model for acute otitis media
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
2003 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 111, no 10, 989-994 p.Article in journal (Refereed) Published
Abstract [en]

To induce acute otitis media in the mouse and to describe the clinical and bacteriological course of the infection, middle ears of BALB/c, Swiss-Webster and C57BL/6 mice were inoculated with Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis. Systemic and local changes were monitored by clinical observations, otomicroscopy, and analysis of bacterial samples from blood and middle ears. Agglutination of mouse erythrocytes by M. catarrhalis was also tested. Depending on bacterial strain, bacterial dose, and mouse strain three responses were identified: acute otitis media, otitis media with serous effusion, or no reaction. BALB/c mice were the most susceptible animals. On day 3, 76% of the BALB/c mice had developed middle ear infection, 50% had a positive middle ear culture, 56% were bacteremic, and 10% had succumbed to a disseminated infection. The local infections lasted approximately a week. Animals which survived recovered without permanent deterioration or otomicroscopically discernible changes. In no case did M. catarrhalis induce a culture-positive middle ear infection, possibly due to an inability to agglutinate the mouse erythrocytes. The mouse model can become a useful tool in studies of pneumococcal and H. influenzae-induced otitis media, but the bacterial dose has to be carefully titrated and adjusted to the chosen mouse strain.

Place, publisher, year, edition, pages
2003. Vol. 111, no 10, 989-994 p.
Keyword [en]
Acute Disease, Animals, Comparative Study, Disease Models; Animal, Haemophilus Infections/etiology, Haemophilus influenzae/pathogenicity, Hemagglutination, In Vitro, Mice, Mice; Inbred BALB C, Mice; Inbred C57BL, Moraxella (Branhamella) catarrhalis/pathogenicity, Otitis Media/*etiology, Pneumococcal Infections/etiology, Research Support; Non-U.S. Gov't, Research Support; U.S. Gov't; P.H.S., Species Specificity, Streptococcus pneumoniae/pathogenicity
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-83397DOI: 10.1034/j.1600-0463.2003.1111012.xPubMedID: 14616553OAI: oai:DiVA.org:uu-83397DiVA: diva2:111305
Available from: 2006-11-02 Created: 2006-11-02 Last updated: 2017-12-14Bibliographically approved

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Melhus, Åsa

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