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Expression of molecular markers for bone formation increases during experimental acute otitis media
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
2001 (English)In: Microbial Pathogenesis, ISSN 0882-4010, Vol. 30, no 3, 111-120 p.Article in journal (Refereed) Published
Abstract [en]

Bony tissues are integral parts of the function of the middle ear and the protection of adjacent vital structures. To explore the reaction of middle ear bone to acute otitis media, rats were challenged with Streptococcus pneumoniae and Haemophilus influenzae. Local changes were monitored for up to 1 month. After reverse transcription, competitive polymerase chain reaction was used to determine the expression levels of two molecular markers of bone formation, osteocalcin and procollagen I, and the two cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha, in the bone. Middle ear bone responded rapidly to bacterial challenge, and the reaction depended upon the causative agent. On day 1, IL-6 and TNF-alpha transcripts were detected in the bone from all middle ears. After a short period of decreased expression of osteocalcin, during which the otitis diagnosis could not be made clinically, the levels of bone formation markers increased dramatically. The maximum levels of these markers were reached on days 6 and 14 for animals challenged with H. influenzae and pneumococci, respectively. Infections induced by pneumococci had a longer duration, and after the initial phase the production of osteocalcin and procollagen transcript were significantly higher in the pneumococcus-infected animals. The results indicate that even in an uncomplicated infection, the bone of the bulla reacts to an acute otitis media with a short period of inhibited osteoblast activity followed by a longer period of new bone formation.

Place, publisher, year, edition, pages
2001. Vol. 30, no 3, 111-120 p.
Keyword [en]
Acute Disease, Animals, Bone Remodeling/genetics/physiology, Disease Models; Animal, Ear; Middle/microbiology/pathology, Genetic Markers, Haemophilus Infections/microbiology/pathology/*physiopathology, Haemophilus influenzae, Humans, Interleukin-6/genetics/metabolism, Male, Osteocalcin/genetics/metabolism, Otitis Media/microbiology/pathology/*physiopathology, Pneumococcal Infections/microbiology/pathology/*physiopathology, Procollagen/genetics/metabolism, Rats, Rats; Sprague-Dawley, Research Support; Non-U.S. Gov't, Research Support; U.S. Gov't; Non-P.H.S., Research Support; U.S. Gov't; P.H.S., Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha/genetics/metabolism
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-83408DOI: 10.1006/mpat.2000.0414PubMedID: 11273736OAI: oai:DiVA.org:uu-83408DiVA: diva2:111316
Available from: 2006-11-02 Created: 2006-11-02Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=11273736&dopt=Citation

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Melhus, Åsa
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