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Blood culture bottles for transportation and recovery of anaerobic bacteria from non-blood samples
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
2000 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, Vol. 108, no 6, 453-458 p.Article in journal (Refereed) Published
Abstract [en]

Using bacterial suspensions as simulated non-blood specimens, the capacity of three different BacT/ Alert blood culture bottles for the transportation and recovery of anaerobic bacteria with different sensitivity to air was evaluated. To better assess the performance of the BacT/Alert bottles, three other liquid media specially designed for anaerobes were included in the study. Attention was paid to recovery rates in relation to species, initial bacterial concentration, and time needed for detection. Of the BacT/Alert blood culture bottles, the anaerobic FAN bottle yielded the highest recovery rates, but its performance was limited compared with chopped meat broth in tubes. This broth allowed detection of all the tested species within 48 h. Since collection and transportation of anaerobic bacteria are of major importance for a reliable culture result, improvements are necessary.

Place, publisher, year, edition, pages
2000. Vol. 108, no 6, 453-458 p.
Keyword [en]
Anaerobiosis, Bacteria; Anaerobic/*isolation & purification, Bacteriological Techniques, Blood Specimen Collection/*instrumentation, Culture Media, Humans, Research Support; Non-U.S. Gov't, Specimen Handling/instrumentation/*methods
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-83410DOI: 10.1034/j.1600-0463.2000.108006453.xPubMedID: 11028809OAI: oai:DiVA.org:uu-83410DiVA: diva2:111318
Available from: 2006-11-02 Created: 2006-11-02Bibliographically approved

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Melhus, Åsa
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