Experimental recurrent otitis media induced by Haemophilus influenzae: protection and serum antibodies
1995 (English)In: American Journal of Otolaryngology, ISSN 0196-0709, Vol. 16, no 6, 383-390 p.Article in journal (Refereed) Published
PURPOSE: To study whether acute otitis media caused by encapsulated or nontypeable Haemophilus influenzae confers cross-reactive protective immunity in an animal model system and to explore the possible involvement of various humoral specific antibodies in protection. MATERIALS AND METHODS: Rats were intrabullarly challenged with H influenzae type b and two different nontypeable H influenzae strains. One month after the initial infection, the animals were rechallenged ipsilaterally or contralaterally with either a homologous or heterologous strain, and the susceptibility to reinfection was investigated by otomicroscopy. RESULTS: The animals challenged and rechallenged with the type b strain were well-protected ipsilaterally and contralaterally, while the protection after homologous rechallenge with a nontypeable strain was partial in the ipsilateral ear and very poor in the contralateral ear. Middle ears previously infected with a nontypeable strain remained fully susceptible to infections with heterologous strains, but there was an indication of cross-protection in the animal groups where the first episode of acute otitis media was caused by type b and the second by a nontypeable strain. Using the Western blot technique and an enzyme linked immunosorbant assay, the serological response to different outer membrane proteins, especially protein D, of H influenzae during and after middle ear infection were investigated. The serological response from the type b infected animals were generally more distinct, while the antibody levels against protein D were lower in these groups compared with the groups infected with nontypeable strains. CONCLUSIONS: These data indicate that H influenzae type-b-induced experimental otitis media results in a better protection than a nontypeable-induced, and H influenzae b confers a cross protection.
Place, publisher, year, edition, pages
1995. Vol. 16, no 6, 383-390 p.
Animals, Antibodies; Bacterial/biosynthesis/*blood, Bacterial Outer Membrane Proteins/immunology, Bacterial Proteins, Blotting; Western, Carrier Proteins/immunology, Comparative Study, Cross Reactions, Disease Models; Animal, Disease Susceptibility, Enzyme-Linked Immunosorbent Assay, Haemophilus Infections/*immunology, Haemophilus influenzae/classification/*immunology, Immunization, Immunoglobulin D/immunology, Lipoproteins/immunology, Male, Microscopy, Otitis Media/*immunology/*microbiology, Rats, Rats; Sprague-Dawley, Recurrence, Research Support; Non-U.S. Gov't, Serotyping
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-83430DOI: 10.1016/0196-0709(95)90075-6PubMedID: 8572254OAI: oai:DiVA.org:uu-83430DiVA: diva2:111338