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Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
CSIC, CIAL, Inst Food Sci Res, Lab Food, Nicolas Cabrera 9, Madrid 28049, Spain..
CSIC, CIAL, Inst Food Sci Res, Mol Nutr & Metab, Nicolas Cabrera 9, Madrid 28049, Spain..
Miguel Herndndez Univ, Inst Mol & Cellular Biol, Avda Univ S-N, Elche 03202, Spain..
Miguel Herndndez Univ, Inst Mol & Cellular Biol, Avda Univ S-N, Elche 03202, Spain..
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2017 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1499, 90-100 p.Article in journal (Refereed) Published
Abstract [en]

The antiproliferative activity of Rosemary (Rosmarinus officinalis) has been widely studied in different in vitro and in vivo models, which demonstrate that rosemary extracts inhibit the cellular proliferation due to its ability to interact with a wide spectrum of molecular targets. However, a comprehensive proteomics study in vivo has not been carried out yet. In the present work, the effects of rosemary extract on xenograft tumor growth has been studied and, for the first time, a shotgun proteomic analysis based on nano-LC-MS/MS together with stable isotope dimethyl labeling (DML) has been applied to investigate the global protein changes in vivo. Our results show that the daily administration of a polyphenol-enriched rosemary extract reduces the progression of colorectal cancer in vivo with the subsequent deregulation of 74 proteins. The bioinformatic analysis of these proteins indicates that the rosemary extract mainly alters the RNA Post-Transcriptional Modification, the Protein Synthesis and the Amino Acid Metabolism functions and suggests the inactivation of the oncogene MYC. These results demonstrate the high utility of the proposed analytical methodology to determine, simultaneously, the expression levels of a large number of protein biomarkers and to generate new hypothesis about the molecular mechanisms of this extract in vivo.

Place, publisher, year, edition, pages
2017. Vol. 1499, 90-100 p.
Keyword [en]
Colon cancer, Dimethyl labeling, Mass spectrometry, Rosemary extract, Quantitative proteomics, Xenograft mice
National Category
Biochemistry and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-325693DOI: 10.1016/j.chroma.2017.03.072ISI: 000401394200009PubMedID: 28389096OAI: oai:DiVA.org:uu-325693DiVA: diva2:1116069
Funder
Swedish Research Council, 2011-4423Swedish Research Council, 2015-4870
Available from: 2017-06-27 Created: 2017-06-27 Last updated: 2017-06-27Bibliographically approved

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Artemenko, Konstantin A.Bergquist, Jonas
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