Labeling of aliphatic carboxylic acids using [11C]carbon monoxide
2006 (English)In: Nature Protocols, ISSN 1754-2189, Vol. 1, no 2, 798-802 p.Article in journal (Refereed) Published
Here we present a protocol for labeling aliphatic carboxylic acids with the positron-emitting radionuclide 11C (t1/2 = 20.4 min) at the carboxyl position using [11C]carbon monoxide via photoinitiated free radical-mediated carbonylation. A solution of an alkyl iodide in a homogenous binary organic solvent-water mixture is introduced into a high-pressure photochemical reactor containing [11C]carbon monoxide. Then the reactor contents are pressurized to 40 MPa and irradiated with ultraviolet light for 6 min. The labeled product is purified using HPLC. All manipulations with radioactivity including the labeling synthesis are carried out on an automated Synthia system. In a typical case, 3.19 GBq of purified [1-11C]1,10-decanedicarboxylic acid (with a specific radioactivity of 188 GBq/μmol) can be obtained within 35 min after the end of a 10-μAh bombardment. Compared to previous labeling methods, this protocol is compatible with a wider range of functional groups, utilizes less-sensitive precursors, and is less subject to isotopic dilution.
Place, publisher, year, edition, pages
2006. Vol. 1, no 2, 798-802 p.
IdentifiersURN: urn:nbn:se:uu:diva-83892DOI: 10.1038/nprot.2006.112ISI: 000251002500039PubMedID: 17406310OAI: oai:DiVA.org:uu-83892DiVA: diva2:111800