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Testing the specificity of the pBAD arabinose reporter
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2017 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The project highlights Salmonella enterica subspecies enterica serovar Typhimurium (S. Tm)'s ability to metabolize simple sugars released from dead commensal bacteria, by using the pBAD (araBAD promoter) system as a reporter of L-arabinose availability. Using bioinformatics and homology of conserved L-arabinose transporter genes shared in Escherichia coli K12 (E. coli) and S. Tm, we aimed to create a S. Tm mutant strain unable to obtain L-arabinose from it environment. During the projects course of time it was discovered that L-arabinose transporters are not a shared gene trait between E. coli and S. Tm, and that putative L-arabinose transporter orthologues may exists in the S. Tm genome.

Place, publisher, year, edition, pages
2017. , 31 p.
National Category
Microbiology Immunology Bioinformatics and Systems Biology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-325983OAI: oai:DiVA.org:uu-325983DiVA: diva2:1118023
External cooperation
Institute of Microbiology at ETH Hönggerberg, Zürich
Educational program
Bachelor Programme in Biology / Molecular Biology
Presentation
2017-06-09, ETH Hönggerberg, Zürich, 08:30 (English)
Supervisors
Available from: 2017-06-30 Created: 2017-06-29 Last updated: 2017-06-30Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2018-06-30 15:30
Available from 2018-06-30 15:30

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