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A subcellular map of the human proteome
KTH Royal Inst Technol, Sch Biotechnol, Sci Life Lab, SE-17121 Stockholm, Sweden..
KTH Royal Inst Technol, Sch Biotechnol, Sci Life Lab, SE-17121 Stockholm, Sweden..
KTH Royal Inst Technol, Sch Biotechnol, Sci Life Lab, SE-17121 Stockholm, Sweden..
KTH Royal Inst Technol, Sch Biotechnol, Sci Life Lab, SE-17121 Stockholm, Sweden..
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2017 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 356, no 6340, eaal332Article in journal (Refereed) Published
Abstract [en]

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

Place, publisher, year, edition, pages
AMER ASSOC ADVANCEMENT SCIENCE , 2017. Vol. 356, no 6340, eaal332
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-326232DOI: 10.1126/science.aal3321ISI: 000401957900032OAI: oai:DiVA.org:uu-326232DiVA: diva2:1120171
Funder
Knut and Alice Wallenberg FoundationWellcome trust, 108441/Z/15/ZScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Available from: 2017-07-05 Created: 2017-07-05 Last updated: 2017-07-05Bibliographically approved

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Lindskog, CeciliaSjöstedt, EvelinaPontén, Fredrik

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