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Barriers to Dissemination of Antibiotic Resistance Genes from Wastewater
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Antibiotic resistance genes can spread between environments and eventually reach pathogenic bacteria through horizontal gene transfer. Wastewater treatment plants (WWTPs) receive water from various sources and contain a mixture of human pathogens and antibiotic waste. Therefore, WWTPs are believed to be a major source of antibiotic resistant gene dissemination. However, it has been demonstrated that even if resistance genes are abundant in WWTPs, only a few are disseminated to other environments. In this study, we expressed 58 genes from a WWTP in Denmark. The genes are known to give resistance towards different antibiotics in Escherichia coli. The level of resistance to antibiotics as well as the fitness cost was analysed to evaluate if these factors could constitute barriers to gene dissemination. We found that both the level of resistance and the fitness cost conferred by the antibiotic resistance WWTP genes were highly variable. Moreover, an increased level of resistance was not necessarily correlated with a fitness cost. This study could not conclude that the level of resistance or the fitness cost could explain the barrier to dissemination of these genes. 

Place, publisher, year, edition, pages
2017. , 28 p.
Keyword [en]
antibiotic, wastewater, resistance, horizontal gene transfer
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-326657OAI: oai:DiVA.org:uu-326657DiVA: diva2:1127789
Educational program
Master Programme in Infection Biology
Supervisors
Examiners
Available from: 2017-11-02 Created: 2017-07-19 Last updated: 2017-11-02Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2018-08-01 15:43
Available from 2018-08-01 15:43

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Department of Medical Biochemistry and Microbiology
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