uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Glyphosate´s effect on the developing brain
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 HE creditsStudent thesis
Abstract [en]

Glyphosate is the most widely applied pesticide worldwide and is the primary active ingredient in Roundup ® (Monsanto Company, St. Louis, MO). Glyphosate mechanism of action and effect on humans is still being investigated. It has been shown that pesticide exposure could contribute to develop neurodegenerative disorders by inducing oxidative damage, increased risk to develop Parkinson´s and Alzheimer´s disease, and might induce age-related neurodegeneration.

The aim of this study was to investigate glyphosates long-term affect on Hippocampus (HC) and Substantia nigra (SN) after neonatal and postnatal exposure. MALDI mass spectrometry (MS) imaging was used to investigate peptide expression and changes in between groups.

The aim of the second part was to set up a pilot-experiment to use the ChiP printer for coating MALDI slides with purpose for future MALDI MS imaging experiment on glyphosate and Roundup® exposed cell cultures.

The results indicate that glyphosate has a significant affect on SN with 188 significant peaks, while HC showed no significant peaks between the groups. Nigral glyphosate exposure resulted in a significant (P<0.05) peak intensity reduction of Des-Tyr (50%), a-Neo (50%) and Dyn-B (57%). The overall quality of the data was evaluated by validation on variability within groups and group variance.

Furthermore, the pilot experiment showed that the ChiP printer could be used as coating instrument for coating MALDI slides for MALDI MS imaging. Neuronal localization and density can be controlled and matrix for MS analysis can be added on the exact same position as the coating material by re-using the print template.

Place, publisher, year, edition, pages
2017. , 29 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-328440OAI: oai:DiVA.org:uu-328440DiVA: diva2:1135449
Supervisors
Examiners
Available from: 2017-09-05 Created: 2017-08-23 Last updated: 2017-09-05Bibliographically approved

Open Access in DiVA

No full text

By organisation
Department of Pharmaceutical Biosciences
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 136 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf