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Localization and Characterization of the Enkephalin and Dynorphin expressing interneuron populations in the mouse spinal cord and validation of DREADD expression induced by virus injection
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. (Sensory circuits)
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The spinal cord dorsal horn is the location where primary afferent sensory neurons relay signals from periphery to the central nervous system. This location is of high interest for pain and itch related research because it is here that incoming signals are modulated by dorsal horn interneurons, highly heterogeneous populations that up/down regulate signals before conduction to sensory modules in the brain. This primary modulation is involved in diseases such as allodynia, hyperalgesia and chronic pruritus. We use fluorescence immunohistochemistry to determine the dorso-ventral distribution and neurochemical phenotype of two opioid signalling populations; enkephalin and dynorphin positive interneurons defined by the activity of respective promotor. The populations were found to be fairly similar in both distribution and transmitter phenotype. We also validated Cre driven expression of inhibitory and excitatory DREADDs to a control based on a mCherry proxy.

Place, publisher, year, edition, pages
2017. , 19 p.
Keyword [en]
DREADD, pain, itch, spinal cord, enkephalin, dynorphin
National Category
Genetics Neurosciences
Identifiers
URN: urn:nbn:se:uu:diva-328492OAI: oai:DiVA.org:uu-328492DiVA: diva2:1135756
Educational program
Master Programme in Biology
Supervisors
Available from: 2017-08-30 Created: 2017-08-24 Last updated: 2017-08-30Bibliographically approved

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