uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Artifact reduction in the CSPAD detectors used for LCLS experiments
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division of Scientific Computing. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computational Science. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-0458-6902
2017 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 24, p. 1092-1097Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2017. Vol. 24, p. 1092-1097
National Category
Biophysics
Identifiers
URN: urn:nbn:se:uu:diva-328543DOI: 10.1107/S160057751701058XISI: 000408902800025PubMedID: 28862634OAI: oai:DiVA.org:uu-328543DiVA, id: diva2:1136172
Projects
eSSENCEAvailable from: 2017-07-18 Created: 2017-08-25 Last updated: 2019-01-14Bibliographically approved
In thesis
1. Statistical processing of Flash X-ray Imaging of protein complexes
Open this publication in new window or tab >>Statistical processing of Flash X-ray Imaging of protein complexes
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Flash X-ray Imaging (FXI) at X-ray Free Electron Lasers (XFELs) is a promising technique that permits the investigation of the 3D structure of molecules without the need for crystallization, by diffracting on single individual sample particles.

In the past few years, some success has been achieved by using FXI on quite large biological complexes (40 nm-1 μm in diameter size). Still, the desired dream-goal of imaging a single individual of a molecule or a protein complex (<15 nm in diameter size) has not been reached yet. The main issue that prevented us from a complete success has been the low signal strength, almost comparable to background noise. That is particularly true for experiments performed at the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS).

In this thesis, we provide a brief review of the CXI instrument (focusing on experiments there performed) and present a statistical method to deal with low signal-to-noise ratios. We take into account a variety of biological particles, showing the benefits of estimating a background model from sample data and using that for processing said data. Moreover, we present the results of some computer simulations in order to explore the limits and potentials of the proposed approach.

Last, we show another method (named COACS) that, being fed with the previous findings from the background model, helps obtaining clearer results in the phase retrieval problem.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2019. p. 88
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1764
Keywords
X-ray, XFEL, single particle, hit-finder, statistical hit-finder, single protein, protein complex, RNA polymerase II, FEL, free-electron laser, FXI, Flash X-ray Imaging, CXI, CSPAD, x-ray imaging, COACS
National Category
Biological Sciences Physical Sciences
Research subject
Physics with specialization in Biophysics
Identifiers
urn:nbn:se:uu:diva-372987 (URN)978-91-513-0554-7 (ISBN)
Public defence
2019-03-06, C8:301, BMC, Husargatan 3, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2019-02-08 Created: 2019-01-14 Last updated: 2019-02-18

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Pietrini, AlbertoNettelblad, Carl

Search in DiVA

By author/editor
Pietrini, AlbertoNettelblad, Carl
By organisation
Molecular biophysicsDivision of Scientific ComputingComputational ScienceScience for Life Laboratory, SciLifeLab
In the same journal
Journal of Synchrotron Radiation
Biophysics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 229 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf