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Neuropeptide imaging in rat spinal cord with MALDI-TOF MS: Method development for the application in pain-related disease studies
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
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2017 (English)In: European journal of mass spectrometry, ISSN 1469-0667, E-ISSN 1751-6838, Vol. 23, no 3, 105-115 p.Article in journal (Refereed) Published
Abstract [en]

Spinal cord as a connection between brain and peripheral nervous system is an essential material for studying neural transmission, especially in pain-related research. This study was the first to investigate pain-related neuropeptide distribution in rat spinal cord using a matrix-assisted laser desorption ionization-time of flight imaging mass spectrometry (MALDI TOF MS) approach. The imaging workflow was evaluated and showed that MALDI TOF MS provides efficient resolution and robustness for neuropeptide imaging in rat spinal cord tissue. The imaging result showed that in naive rat spinal cord the molecular distribution of haeme, phosphatidylcholine, substance P and thymosin beta 4 were well in line with histological features. Three groups of pain-related neuropeptides, which are cleaved from prodynorphin, proenkephalin and protachykinin-1 proteins were detected. All these neuropeptides were found predominantly localized in the dorsal spinal cord and each group had unique distribution pattern. This study set the stage for future MALDI TOF MS application to elucidate signalling mechanism of pain-related diseases in small animal models.

Place, publisher, year, edition, pages
2017. Vol. 23, no 3, 105-115 p.
Keyword [en]
Matrix-assisted laser desorption ionization-time of flight imaging mass spectrometry, neuropathic pain, neuropeptide imaging, rat spinal cord
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:uu:diva-330364DOI: 10.1177/1469066717703272ISI: 000405717700004PubMedID: 28657437OAI: oai:DiVA.org:uu-330364DiVA: diva2:1145270
Funder
Swedish Research Council, 621-2011-4423, 2015-4870
Available from: 2017-09-28 Created: 2017-09-28 Last updated: 2017-10-24Bibliographically approved

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Sui, PingWatanabe, HiroyukiArtemenko, KonstantinBakalkin, GeorgyAndersson, MalinBergquist, Jonas
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