uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
IL-33 stimulates the release of procoagulant microvesicles from human monocytes and differentially increases tissue factor in human monocyte subsets
Med Univ Vienna, Dept Internal Med 2, Vienna, Austria..
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Coagulation and inflammation science.
Med Univ Vienna, Dept Internal Med 1, Clin Div Hematol & Hemostaseol, Vienna, Austria..
Med Univ Vienna, Dept Internal Med 2, Vienna, Austria..
Show others and affiliations
2017 (English)In: Thrombosis and Haemostasis, ISSN 0340-6245, Vol. 117, no 7, 1379-1390 p.Article in journal (Refereed) Published
Abstract [en]

Monocytes and monocyte-derived microvesicles (MVs) are the main source of circulating tissue factor (TF). Increased monocyte TF expression and increased circulating levels of procoagulant MVs contribute to the formation of a prothrombotic state in patients with cardiovascular disease. Interleukin (IL)-33 is a pro-inflammatory cytokine involved in atherosclerosis and other inflammatory diseases, but its role in regulating thrombosis is still unclear. The aim of the present study was to investigate the effects of IL-33 on the procoagulant properties of human monocytes and monocyte-derived MVs. IL-33 induced a time- and concentration-dependent increase of monocyte TF mRNA and protein levels via binding to the ST2-receptor and activation of the NF-kappa B-pathway. The IL-33 treated monocytes also released CD14+TF+ MVs and IL-33 was found to increase the TF activity of both the isolated monocytes and monocyte-derived MVs. The monocytes were classified into subsets according to their CD14 and CD16 expression. Intermediate monocytes (IM) showed the highest ST2 receptor expression, followed by non-classical monocytes (NCM), and classical monocytes (CM). IL-33 induced a significant increase of TF only in the IM (p<0.01), with a tendency in NCM (p=0.06), but no increase was observed in CM. Finally, plasma levels of IL-33 were positively correlated with CD14+TF+ MVs in patients undergoing carotid endarterectomy (r=0.480; p=0.032; n=20). We hereby provide novel evidence that the proinflammatory cytokine IL-33 induces differential TF expression and activity in monocyte subsets, as well as the release of procoagulant MVs. In this manner, IL-33 may contribute to the formation of a prothrombotic state characteristic for cardiovascular disease.

Place, publisher, year, edition, pages
SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN , 2017. Vol. 117, no 7, 1379-1390 p.
Keyword [en]
Interleukin-33, tissue factor, monocyte subsets, microvesicles, thrombosis
National Category
Hematology Cardiac and Cardiovascular Systems
Identifiers
URN: urn:nbn:se:uu:diva-330042DOI: 10.1160/TH16-10-0784ISI: 000404671700018PubMedID: 28492698OAI: oai:DiVA.org:uu-330042DiVA: diva2:1145355
Funder
Swedish Research Council, 2012-2579
Available from: 2017-09-28 Created: 2017-09-28 Last updated: 2017-09-28

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Thulin, ÅsaSiegbahn, AgnetaÅberg, Mikael

Search in DiVA

By author/editor
Thulin, ÅsaSiegbahn, AgnetaÅberg, Mikael
By organisation
Science for Life Laboratory, SciLifeLabCoagulation and inflammation scienceUCR-Uppsala Clinical Research CenterDepartment of Medical Biochemistry and MicrobiologyMolecular epidemiologyCardiology
In the same journal
Thrombosis and Haemostasis
HematologyCardiac and Cardiovascular Systems

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 76 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf