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Restrictions in the T-cell repertoire of chronic lymphocytic leukemia: high-throughput immunoprofiling supports selection by shared antigenic elements
CERTH, Inst Appl Biosci, 6th Km Charilaou Thermi Rd, Thessaloniki 57001, Greece.;G Papanicolaou Hosp, Hematol Dept, Thessaloniki, Greece.;G Papanicolaou Hosp, HCT Unit, Thessaloniki, Greece.;Univ Crete, Med Sch, Iraklion, Greece..
CERTH, Inst Appl Biosci, 6th Km Charilaou Thermi Rd, Thessaloniki 57001, Greece..
CERTH, Inst Appl Biosci, 6th Km Charilaou Thermi Rd, Thessaloniki 57001, Greece..
CERTH, Inst Appl Biosci, 6th Km Charilaou Thermi Rd, Thessaloniki 57001, Greece..
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2017 (English)In: Leukemia, ISSN 0887-6924, E-ISSN 1476-5551, Vol. 31, no 7, 1555-1561 p.Article in journal (Refereed) Published
Abstract [en]

Immunoglobulin (IG) gene repertoire restrictions strongly support antigen selection in the pathogenesis of chronic lymphocytic leukemia (CLL). Given the emerging multifarious interactions between CLL and bystander T cells, we sought to determine whether antigen(s) are also selecting T cells in CLL. We performed a large-scale, next-generation sequencing (NGS) study of the T-cell repertoire, focusing on major stereotyped subsets representing CLL subgroups with undisputed antigenic drive, but also included patients carrying non-subset IG rearrangements to seek for T-cell immunogenetic signatures ubiquitous in CLL. Considering the inherent limitations of NGS, we deployed bioinformatics algorithms for qualitative curation of T-cell receptor rearrangements, and included multiple types of controls. Overall, we document the clonal architecture of the T-cell repertoire in CLL. These T-cell clones persist and further expand overtime, and can be shared by different patients, most especially patients belonging to the same stereotyped subset. Notably, these shared clonotypes appear to be disease-specific, as they are found in neither public databases nor healthy controls. Altogether, these findings indicate that antigen drive likely underlies T-cell expansions in CLL and may be acting in a CLL subset-specific context. Whether these are the same antigens interacting with the malignant clone or tumor-derived antigens remains to be elucidated.

Place, publisher, year, edition, pages
2017. Vol. 31, no 7, 1555-1561 p.
National Category
Cancer and Oncology Hematology
Identifiers
URN: urn:nbn:se:uu:diva-330040DOI: 10.1038/leu.2016.362ISI: 000404745300010OAI: oai:DiVA.org:uu-330040DiVA: diva2:1145359
Funder
EU, Horizon 2020, 692298
Available from: 2017-09-28 Created: 2017-09-28 Last updated: 2017-09-28Bibliographically approved

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Stamatopoulos, Kostas

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Experimental and Clinical OncologyDepartment of Immunology, Genetics and Pathology
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