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Results of a collaborative study on DNA identification of aged bone samples
Forens DNA Serv Prague, Prague, Czech Republic.;Charles Univ Prague, Fac Med 2, Prague, Czech Republic..
Univ North Texas, Hlth Sci Ctr, Ctr Human Identificat, Ft Worth, TX 76107 USA.;King Abdulaziz Univ, Ctr Excellence Genom Med Res CEGMR, Jeddah, Saudi Arabia..
Forens DNA Serv Prague, Prague, Czech Republic..
Univ North Texas, Hlth Sci Ctr, Ctr Human Identificat, Ft Worth, TX 76107 USA..
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2017 (English)In: Croatian Medical Journal, ISSN 0353-9504, E-ISSN 1332-8166, Vol. 58, no 3, 203-213 p.Article in journal (Refereed) Published
Abstract [en]

Aim: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples.

Methods: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory.

Results: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality.

Conclusion: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

Place, publisher, year, edition, pages
MEDICINSKA NAKLADA , 2017. Vol. 58, no 3, 203-213 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-330753DOI: 10.3325/cmj.2017.58.203ISI: 000404561800002PubMedID: 28613037OAI: oai:DiVA.org:uu-330753DiVA: diva2:1146687
Available from: 2017-10-03 Created: 2017-10-03 Last updated: 2017-10-03Bibliographically approved

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Allen, MarieBus, Magdalena M.

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Science for Life Laboratory, SciLifeLabDepartment of Immunology, Genetics and PathologyMedicinsk genetik och genomik
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