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Gene expression patterns of GABAA receptor subunits α1 and β2 in the brain and retina of adult zebrafish (Danio rerio Hamilton)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. (Department of Neuroscience)
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

The ionotropic GABAA receptor is one of the most prominent inhibitory neurotransmitter receptor in the central nervous system (CNS). It is clinically important due to its association with benzodiazepine, barbiturates, ethanol, and general anesthetics. The arrangement and composition of the subunits comprising the GABAA receptors affect its pharmacological properties. The zebrafish has at least twenty-two different subunits which may arise from 8 gene families (α1-6b, β1-4, γ1-3, δ, ε, π, ρ1-3a, ζ). This thesis focuses in depth on methods utilized, ie RNA extraction,  probe synthesis, and in situ hybridization. Moreover, the gene expression of the α1 and β2 subunits in the brain and retinas of the adult zebrafish was analyzed. In the brain, we found expression of the α1 subunit in the dorsal telencephalic area, ventral telencephalic area, and the preglomerular nucleus and the β2 subunit in the periventricular gray zone, torus longitudinalis and immediate area surrounding the vulva cerebelli, corpus mamillare, lateral recess, and areas of the caudal zone of periventricular hypothalamus. In the retinas, the α1 and β2 antisense probe showed specific staining in the outer nuclear layer and inner nuclear layer. We controlled using the sense probe of each subunit. The α1 sense probe did not show staining in the brain or retina, but the β2 probe did.

Place, publisher, year, edition, pages
2017. , p. 34
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-331015OAI: oai:DiVA.org:uu-331015DiVA, id: diva2:1148105
Educational program
Master Programme in Biology
Examiners
Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-10-10Bibliographically approved

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