uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Elemene inhibits the migration and invasion of 4T1 murine breast cancer cells via heparanase
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, 23 Back Rd Art Gallery, Beijing 100010, Peoples R China..
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, 23 Back Rd Art Gallery, Beijing 100010, Peoples R China..
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, 23 Back Rd Art Gallery, Beijing 100010, Peoples R China..
Capital Med Univ, Beijing Hosp Tradit Chinese Med, Dept Oncol, 23 Back Rd Art Gallery, Beijing 100010, Peoples R China..
Show others and affiliations
2017 (English)In: Molecular Medicine Reports, ISSN 1791-2997, E-ISSN 1791-3004, Vol. 16, no 1, 794-800 p.Article in journal (Refereed) Published
Abstract [en]

Elemene (ELE), a natural plant drug extracted from Curcumae Rhizoma, has been widely used for cancer treatment in China for more than 20 years. Although it is reported to be a broad-spectrum anticancer drug, the mechanism underlying the action of ELE in the treatment of breast cancer remains to be fully elucidated. Heparanase, a mammalian endo-D-glucuronidase, is involved in degradation of the extracellular matrix (ECM), and thus promotes tumor progression and metastasis. The downregulation of heparanase can effectively reduce tumor malignant behaviors. In the present study, the inhibitory effects of ELE were evaluated in breast cancer cells using a Cell Counting kit 8 assay. The migratory and invasive capabilities of cancer cells were investigated using a wound healing assay, real-time cell analysis and a Transwell assay. In addition, western blot analysis was used to assess alterations in the expression levels of key proteins. The present results confirmed the antiproliferative and antimetastatic effects of ELE, using low-molecular weight heparin (LMWH) as a positive control. In addition, ELE was demonstrated to downregulate the expression of heparanase, and decrease the phosphorylation of extracellular signal-regulated kinase and AKT. These findings suggested that ELE may be a promising agent targeting heparanase in the treatment of breast cancer.

Place, publisher, year, edition, pages
2017. Vol. 16, no 1, 794-800 p.
Keyword [en]
elemene, invasion, migration, heparanase, extracellular regulated kinase, AKT
National Category
Cancer and Oncology Basic Medicine
Identifiers
URN: urn:nbn:se:uu:diva-330011DOI: 10.3892/mmr.2017.6638ISI: 000405074100104PubMedID: 28560389OAI: oai:DiVA.org:uu-330011DiVA: diva2:1148530
Available from: 2017-10-11 Created: 2017-10-11 Last updated: 2017-10-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedhttps://www.spandidos-publications.com/mmr/16/1/794

Authority records BETA

Li, Jin-Ping

Search in DiVA

By author/editor
Li, Jin-Ping
By organisation
Department of Medical Biochemistry and MicrobiologyScience for Life Laboratory, SciLifeLab
In the same journal
Molecular Medicine Reports
Cancer and OncologyBasic Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 37 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf