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The relative importance of richness and BCC for DOC degradation
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology. Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology. Catalan Institute for Water Research (ICRA).
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

The importance of biodiversity has been of primary interest for ecologist the last 20 years, giving rise to biodiversity ecosystem function (BEF) studies. Within the traditional field of ecology reoccurring patterns have emerged but within microbial ecology the importance of species richness for functioning is still poorly understood with few consistent patterns. In this study we examined the effect of species richness for dissolved organic matter degradation in lakes. This was examined within a smaller span of species richness compared to what is typically in microbial BEF experiments. Bacterial communities of reduced species richness were exposed to a range of DOC environments to test if reduced richness changed the functioning of communities and if the effect was similar among DOC environments. This was conducted in a full factorial design of 3 levels, with 6 dilutions, 5 media and 3 inocula resulting in 90 treatments. Overall, richness and community composition appeared to have effects on DOC degradation, but these effects were minor compared to the variation caused by the different DOC sources. Further, the importance of species richness varied among media and, thus, the chemical complexity of the environment influenced the biodiversity-ecosystem functioning relationship. 

National Category
Biological Sciences
Research subject
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-331693OAI: oai:DiVA.org:uu-331693DiVA: diva2:1149750
Available from: 2017-10-17 Created: 2017-10-17 Last updated: 2017-10-23
In thesis
1. Extent and limitations of functional redundancy among bacterial communities towards dissolved organic matter
Open this publication in new window or tab >>Extent and limitations of functional redundancy among bacterial communities towards dissolved organic matter
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

One of the key processes in the carbon cycle on our planet is the degradation of dissolved organic matter (DOM) in aquatic environments. The use of organic matter by bacteria links energy from DOM to higher trophic levels of the ecosystem when bacteria are consumed by other organisms. This is referred to as the microbial loop. In this thesis I examined if the communities were functionally redundant in their ability to utilize organic matter, or if variation in bacterial composition and richness is of importance. To test this overarching question several experiments were conducted that include methods such as illumina sequencing of the 16S rRNA gene for taxonomic identification of bacterial communities, flow cytometry to follow the growth of communities and spectroscopic measurement to describe the composition of the organic matter pool. Initially we demonstrated how to optimally sterilize organic matter for experimental studies in order to preserve its natural complexity. In further experiments we found that bacterial communities are redundant in their utilization of organic matter and can maintain optimal performance towards a range of organic matter pools. Related to this we found that pre-adaptation to organic matter played a small role as communities performed equally well regardless of their environmental history. We saw a small effect of richness and composition of bacterial communities on the efficiency of organic matter use, but conclude that this is of minor importance relative to abiotic factors. Still, we also show that organic matter can put strong selection pressure on bacterial communities with regards to richness and composition. Additionally we found that the supply rate of a carbon compound greatly influenced the energy utilization of the compound, i.e. a higher growth rate can be maintained if substrate is delivered in pulses relative to a continuous flow. Finally we conclude that the variation in bacterial communities is unlikely to have a major influence on carbon cycling in boreal lakes, but to enable a finer understanding, the genetics underlying the carbon utilization needs to be further explored. 

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2017. 41 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1578
Keyword
Dissolved organic matter, BCC, biodiversity, functional redundancy
National Category
Natural Sciences
Research subject
Microbiology
Identifiers
urn:nbn:se:uu:diva-331772 (URN)978-91-513-0112-9 (ISBN)
Public defence
2017-12-01, Friessalen, Norbyvägen 18, Uppsala, 09:00 (English)
Opponent
Supervisors
Available from: 2017-11-08 Created: 2017-10-17 Last updated: 2017-11-08Bibliographically approved

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