uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Spatially resolved transcriptome profiling in model plant species
KTH Royal Inst Technol, Sch Biotechnol, Div Gene Technol, Sci Life Lab, S-17165 Solna, Sweden.;Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, S-17165 Solna, Sweden..ORCID iD: 0000-0003-0738-1574
Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90736 Umea, Sweden..
Show others and affiliations
2017 (English)In: NATURE PLANTS, ISSN 2055-026X, Vol. 3, no 6, 17061Article in journal (Refereed) Published
Abstract [en]

Understanding complex biological systems requires functional characterization of specialized tissue domains. However, existing strategies for generating and analysing high-throughput spatial expression profiles were developed for a limited range of organisms, primarily mammals. Here we present the first available approach to generate and study highresolution, spatially resolved functional profiles in a broad range of model plant systems. Our process includes highthroughput spatial transcriptome profiling followed by spatial gene and pathway analyses. We first demonstrate the feasibility of the technique by generating spatial transcriptome profiles from model angiosperms and gymnosperms microsections. In Arabidopsis thaliana we use the spatial data to identify differences in expression levels of 141 genes and 189 pathways in eight inflorescence tissue domains. Our combined approach of spatial transcriptomics and functional profiling offers a powerful new strategy that can be applied to a broad range of plant species, and is an approach that will be pivotal to answering fundamental questions in developmental and evolutionary biology.

Place, publisher, year, edition, pages
2017. Vol. 3, no 6, 17061
National Category
Genetics
Identifiers
URN: urn:nbn:se:uu:diva-331942DOI: 10.1038/nplants.2017.61ISI: 000406036800002PubMedID: 28481330OAI: oai:DiVA.org:uu-331942DiVA: diva2:1152345
Funder
Knut and Alice Wallenberg FoundationSwedish Research CouncilCarl Tryggers foundation
Available from: 2017-10-24 Created: 2017-10-24 Last updated: 2017-10-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Giacomello, StefaniaReimegård, Johan
By organisation
Molecular EvolutionScience for Life Laboratory, SciLifeLab
Genetics

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 23 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf