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Investigating Protein Throughput, Vmax Values and Virus Removal Efficiency of the Paper Based Mille-Feuille Filter
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0002-3828-4083
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0002-8105-2317
2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

In this work we present Vmax values and throughput for the nanocellulose-based Mille-feuille virus removal filter paper as well as evaluate the affinity of different proteins for the cellulose surface using quartz crystal microbalance (QCMB). The Minute Virus of Mice (MVM) virus removal result for the Mille-feuille filter is also presented. The nanocellulose-based Mille-feuille filter is a non-woven, ┬Ám-thick filter paper with a narrow and tailorable pore size distribution, highly suitable for removing viruses of all sizes, including the worst case model Minute Virus of Mice (MVM). It is produced by hot-pressing method rather than by phase-inversion which is otherwise used to manufacture industrial analogues. Robust and cost-efficient virus removal processes are in high demand for the biotechnology industry, especially for the production of monoclonal antibodies derived from mammalian cell lines, therapeutic proteins derived from human plasma, and cell culture media. Non-woven filters have the advantage in contrast to regular phase-inversion made filters of a much higher porosity. For instance, the Mille-feuille filter has a porosity of 42% compared to 10-15% of the functional skin layer in asymmetric virus removal filters used today. In theory a higher porosity will result in higher flow rates. However, it also means that there is a larger filter area exposed to filtrate. Therefore investigating the surface affinity for proteins with different hydrophobicity and charge using QCMB is of great importance to be able to predict protein filtration behaviour. The presented work is a part of continuous efforts to develop a new class of highly efficient and affordable virus removal filters.

Place, publisher, year, edition, pages
2017.
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:uu:diva-332593OAI: oai:DiVA.org:uu-332593DiVA: diva2:1153519
Conference
ACS National Meeting - San Francisco 2017 253rd American Chemical Society National Meeting & Expo
Funder
Knut and Alice Wallenberg Foundation
Available from: 2017-10-30 Created: 2017-10-30 Last updated: 2017-11-02Bibliographically approved

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CiteExportLink to record
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