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Dynamics of insertion sequence element IS629 inactivation of verotoxin 2 genes in Escherichia coli O157:H7
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. SVA, Dept Microbiol, Natl Vet Inst, S-75189 Uppsala, Sweden..
SVA, Dept Microbiol, Natl Vet Inst, S-75189 Uppsala, Sweden.;Swedish Univ Agr Sci, SLU Global Bioinformat Ctr, S-75007 Uppsala, Sweden..
SVA, Dept Microbiol, Natl Vet Inst, S-75189 Uppsala, Sweden..
SVA, Dept Microbiol, Natl Vet Inst, S-75189 Uppsala, Sweden..
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2017 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 364, no 8, fnx074Article in journal (Refereed) Published
Abstract [en]

There are several anecdotal reports of insertion sequence (IS) element inactivation of verotoxin genes among enterohaemorrhagic Escherichia coli of the serotype O157:H7, a pathogen causing severe gastrointestinal disease in infected humans. These insertions can be expected to drastically reduce the virulence of the bacteria. IS element inactivation has been shown to be reversible in model systems, suggesting the possibility of spontaneous restoration of virulence. In this study, traditional and high-throughput sequencing was used to characterise three patterns of IS629 inactivation of verotoxin 2 genes in EHEC O157:H7, caused by insertion or insertion followed by partial deletion. At least one of the patterns of inactivation appears to have persisted several years among cattle O157:H7, indicating it has no major effect on fitness in the animal reservoir. Digital PCR was used to directly quantify the reversal rates of the insertional inactivation of a selected isolate under laboratory conditions. Inserts were found to be absent from in the order of 1/10(5) of individual genomes, with significantly higher loss frequencies observed in cultures under nutrient-poor conditions. We conclude that strains with this type of inactivation found in food or animal samples should be considered a threat to human health, and may pose a challenge for PCR-based detection methods.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 364, no 8, fnx074
Keyword [en]
O157:H7, IS629, VTEC, STEC, verotoxin, digital PCR
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-330064DOI: 10.1093/femsle/fnx074ISI: 000404487400004OAI: oai:DiVA.org:uu-330064DiVA: diva2:1155078
Available from: 2017-11-06 Created: 2017-11-06 Last updated: 2017-11-06Bibliographically approved

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