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A crush on small fungi: An efficient and quick method forobtaining DNA from minute ascomycetes
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology.
Uppsala University, Music and Museums, Museum of Evolution.ORCID iD: 0000-0003-3021-1821
Uppsala University, Music and Museums, Museum of Evolution.
2018 (English)In: Methods in Ecology and Evolution, ISSN 2041-210X, E-ISSN 2041-210X, Vol. 9, no 1, p. 148-158Article in journal (Refereed) Published
Abstract [en]

We have developed a reliable technique for extracting DNA from single microscopic fungal thalli, including efficient cell disruption and transfer of cell content for subsequent polymerase chain reaction (PCR). The technique was primarily developed for members of the ascomycete order Laboulbeniales, which are minute fungi with tough cell walls that are exceedingly difficult to disrupt with standard extraction techniques. Our method makes routine amplification of DNA from single thalli possible, even from small species or poorly developed individuals. DNA release is accomplished in an entirely mechanical manner using an arbor press fitted with custom-made components. This approach has eliminated additional treatment such as laborious freeze-thaw cycles, enzymes, or lysing agents. The overall PCR success rate of 89% is comparable to or better than alternative protocols that make use of substantially larger amounts of fungal tissue. From 97% of the successful PCRs a total of 156 sequences from four gene regions were produced. Being able to restrict DNA extractions to a single thallus is critical to all genetic studies requiring data at the level of individual, e.g. population genetics. As all researchers working with minute uncultivable organisms in many respects face the same problems (effective handling of the material, small quantities of DNA etc.), the methodology described here has a potential to be widely applicable. Necessary custom-made components can be manufactured at fairly low cost by any precision-tool workshop using our detail drawings.

Place, publisher, year, edition, pages
2018. Vol. 9, no 1, p. 148-158
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-334516DOI: 10.1111/2041-210X.12850ISI: 000419821200015OAI: oai:DiVA.org:uu-334516DiVA, id: diva2:1159707
Funder
Helge Ax:son Johnsons stiftelse
Available from: 2017-11-23 Created: 2017-11-23 Last updated: 2018-02-16Bibliographically approved

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Sundberg, HenrikEkman, StefanKruys, Åsa

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