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From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography
Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden..
Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden..
Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden..
Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Hamburg, Germany..
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2017 (English)In: Structure, ISSN 0969-2126, E-ISSN 1878-4186, Vol. 25, no 9, p. 1461-1468Article in journal (Refereed) Published
Abstract [en]

Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RCvir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RCvir serial femtosecond crystallography (SFX) structure from 3.3-angstrom to 2.4-angstrom resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.

Place, publisher, year, edition, pages
CELL PRESS , 2017. Vol. 25, no 9, p. 1461-1468
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-335396DOI: 10.1016/j.str.2017.07.002ISI: 000409243200019PubMedID: 28781082OAI: oai:DiVA.org:uu-335396DiVA, id: diva2:1162834
Funder
Swedish Research Council, 2015-00560, 349-2011-6485Swedish Foundation for Strategic Research , SRL10-0036Knut and Alice Wallenberg Foundation, KAW 2012.0284, KAW 2014.0275, KAW 2012.0106Available from: 2017-12-05 Created: 2017-12-05 Last updated: 2017-12-05Bibliographically approved

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Davidsson, Jan

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