uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Single and mixture impacts of two pyrethroids on damselfly predatory behavior and physiological biomarkers
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Animal ecology.
Univ Leuven, Lab Aquat Ecol Evolut & Conservat, Leuven, Belgium..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Animal ecology.
2017 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 190, p. 70-77Article in journal (Refereed) Published
Abstract [en]

Direct mortality due to toxicity of single pesticide exposure along a concentration gradient, while the most common, is only one important parameter for assessing the effects of pesticide contamination on aquatic ecosystems. Sub-lethal toxicity can induce changes in an organism's behavior and physiology that may have population -level ramifications and consequences for ecosystem health. Additionally, the simultaneous detection of multiple contaminants in monitored watersheds stresses the importance of gaining a greater understanding of the toxicities of combined exposures, particularly at low, environmentally relevant concentrations. Using larvae of the Azure Damselfly (Coenagrion paella), we conducted a combined exposure investigation of two widely-used pyrethroid insecticides presumed to share the same neurotoxic mechanism of action, and estimated their effect on predatory ability, mobility and three physiological biomarkers (Glutathione S-transferase; GST, respiratory electron transport system; ETS, and malondialdehyde; MDA). Deltamethrin exposure (0.065 mu g/L and 0.13 mu g/L) was found to reduce the predatory ability, but it did not affect the larvae's mobility. Esfenvalerate exposure (0.069 mu g/L and 0.13 mu g/L), on the other hand, induced no significant changes in predatory ability or mobility. The decrease in predatory ability after the combination exposure (0.067 mu g/L deltamethrin and 0.12 mu g/L. esfenvalerate) did not significantly differ from the impact of the single deltamethrin exposures. Glutathione-S-transferase was induced after single esfenvalerate exposure and the lower deltamethrin concentration exposure, but seemingly inhibited after exposure to the higher concentration of deltamethrin as well as the combination of both pyrethroids. Our data indicate that sub-lethal exposure to deltamethrin reduces predatory ability and suggest that sub-lethal combined exposure to deltamethrin and esfenvalerate inhibits the GST detoxification pathway. These effects can eventually result in a lower emergence of adults from contaminated ponds.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 190, p. 70-77
Keywords [en]
Pesticides, Combination exposure, behavior, Sub-lethal effects, Aquatic invertebrate, Biomarkers
National Category
Pharmacology and Toxicology Other Biological Topics
Identifiers
URN: urn:nbn:se:uu:diva-335643DOI: 10.1016/j.aquatox.2017.06.025ISI: 000408783600009PubMedID: 28692868OAI: oai:DiVA.org:uu-335643DiVA, id: diva2:1164043
Available from: 2017-12-08 Created: 2017-12-08 Last updated: 2018-06-27Bibliographically approved
In thesis
1. Sub-lethal Effects of Anthropogenic Contaminants on Aquatic Invertebrates
Open this publication in new window or tab >>Sub-lethal Effects of Anthropogenic Contaminants on Aquatic Invertebrates
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Anthropogenic contaminants are considered to play a substantial role in the decline of freshwater invertebrate diversity. Sub-lethal effects of many of these contaminants on behaviour and life-history traits of aquatic invertebrates may contribute to their decline. As contaminants are rarely present in the environment alone, the effects of mixture exposures are highly relevant in assessing the risk these substances pose to the biota. This thesis focuses on sub-lethal effects of exposure to aquatic pollutants, separately and in combination, on fresh-water invertebrates. To investigate the single and combined effects of pesticides, larvae of the midge, Chironomus riparius were exposed to a 1 hour pulse of two neonicotinoids and two pyrethroids.  This short exposure to environmentally relevant concentrations of pesticides decreased the survival and delayed development in C. riparius.  The combination of neonicotinoids and pyrethroids did not produce synergistic effects; however, there was some indication of antagonism. Additionally, larvae of the damselfly, Coenagrion puella, were exposed for 14 hours to two environmentally relevant concentrations of pyrethroid pesticides, alone and in combination. Exposure to the pyrethroid, deltamethrin, reduced the larvae’s predatory ability. Combined exposure to both deltamethrin and esfenvalerate inhibited the Glutathione S-transferase detoxification pathway and may have additive toxic effects on the larvae’s predatory ability. Microplastics are increasingly gaining attention as an aquatic pollutant of major concern with respect to the toxicity of the microplastics themselves as well as their capacity to adsorb persistent organic pollutants like pesticides. To investigate the effects of microplastics and a pyrethroid, alone and in combination, C. riparius larvae were raised in sediment spiked with two sizes of polystyrene-based latex microbeads and an environmentally relevant concentration of esfenvalerate under normal and food-restricted conditions. Exposure to both sizes of microplastics and esfenvalerate lead to equally decreased emergence under food-restricted conditions. Additionally, exposure to esfenvalerate led to decreases in survival when food was scarce that did not occur when microplastics were co-present. Antishistamines are also an emerging aquatic contaminant of concern with very little known about their biological effects on aquatic wildlife. Antihistamines could potentially interfere with the histaminergic pathways and thus affect thermal tolerance and temperature preference in aquatic invertebrates. The freshwater snail, Planorbarius corneus, was exposed for 24 hours to the antihistamine, diphenhydramine. This exposure increased thermal tolerance and righting time, but did not affect temperature preference. The results of the investigation suggest that anthropogenic contaminates alone and/or in combination have sub-lethal effects on life history, behavior and physiology of aquatic invertebrates. Such sub-lethal effects have the potential to affect populations and community structure in the aquatic and terrestrial environment.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2018. p. 70
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1692
Keywords
ecotoxicology, sub-lethal, behavior, biomarkers, thermal tolerance, pesticides, pyrethroids, neonicotinoids, pharmaceuticals, antihistamines, microplastics, mixtures, freshwater, invertebrates, chironomids, damselflies, snails
National Category
Natural Sciences
Research subject
Biology with specialization in Animal Conservation
Identifiers
urn:nbn:se:uu:diva-355236 (URN)978-91-513-0382-6 (ISBN)
Public defence
2018-09-07, Zootissalen, Evolutionsbiologiskt centrum, Norbyvägen 14-18, Uppsala, 10:00 (English)
Opponent
Supervisors
Available from: 2018-08-15 Created: 2018-06-27 Last updated: 2018-08-27

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Kunce, WarrenJohansson, Frank

Search in DiVA

By author/editor
Kunce, WarrenJohansson, Frank
By organisation
Animal ecology
In the same journal
Aquatic Toxicology
Pharmacology and ToxicologyOther Biological Topics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 19 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf