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Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin
Univ Leuven, KU Leuven, Dept Pharmaceut & Pharmacol Sci, Leuven, Belgium..
Univ Leuven, KU Leuven, Dept Pharmaceut & Pharmacol Sci, Leuven, Belgium..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry. Kantisto BV, Baarn, Netherlands..
Univ Leuven, KU Leuven, Dept Pharmaceut & Pharmacol Sci, Leuven, Belgium..
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2017 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 40, no 17, p. 3535-3544Article in journal (Refereed) Published
Abstract [en]

A capillary electrophoresis with capacitively coupled contactless conductivity detection based method for the assay of azithromycin, clarithromycin and clindamycin was optimized and validated in this study. A buffer solution of 20 mM 2-(N-morpholino) ethane sulfonic acid, 40 mM L-histidine and 0.6 mM cetyltrimethylammonium bromide (pH 6.39) was used for the electrophoresis. An uncoated, bare-fused silica capillary (total length 60 cm, effective length 32 cm, 75 mu m id) was used at 25 degrees C. The sample was injected hydrodynamically at 0.5 psi for 5 s. The electrophoresis was conducted at 30 kV in reverse polarity for 6 min with 3 and 2 min of in-between sodium hydroxide (0.1 M) and background electrolyte rinsing, respectively. Ammonium acetate was used as internal standard. This simple and robust method showed reasonable limit of detection and limit of quantitation for azithromycin (0.0125/0.03 mg/mL), clarithromycin (0.017/0.03 mg/mL), and clindamycin (0.038/0.06 mg/mL), with good selectivity, precision both intraday (relative standard deviation <= 1.0%) and interday (relative standard deviation < 3.7%), linearity (R-2 > 0.999) and recovery (99 - 101.7%). The method was successfully applied for the determination of azithromycin, clarithromycin and clindamycin in formulations.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2017. Vol. 40, no 17, p. 3535-3544
Keywords [en]
azithromycin, capacitively coupled contactless conductivity detection, capillary electrophoresis, clarithromycin, clindamycin
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-335630DOI: 10.1002/jssc.201700560ISI: 000409436100018PubMedID: 28683179OAI: oai:DiVA.org:uu-335630DiVA, id: diva2:1165066
Available from: 2017-12-12 Created: 2017-12-12 Last updated: 2017-12-12Bibliographically approved

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Sänger - van de Griend, Cari

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