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Stem cell-mediated functionalization of titanium implants
New York Stem Cell Fdn, Res Inst, 619 West 54th St,3rd Floor, New York, NY 10019 USA..
New York Stem Cell Fdn, Res Inst, 619 West 54th St,3rd Floor, New York, NY 10019 USA..
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.ORCID iD: 0000-0002-7356-3002
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2017 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 28, no 9, article id 133Article in journal (Refereed) Published
Abstract [en]

Prosthetic implants are used daily to treat edentulous people and to restore mobility in patients affected by skeletal defects. Titanium (Ti) is the material of choice in prosthetics, because it can form a stable bond with the surrounding bone following implantation-a process known as osseointegration. Yet, full integration of prosthetic implants takes time, and fails in clinical situations characterized by limited bone quantity and/or compromised regenerative capacity, and in at-risk patients. Intense research efforts are thus made to develop new implants that are cost-effective, safe, and suited to every patient in each clinical situation. In this study, we tested the possibility to functionalize Ti implants using stem cells. Human induced pluripotent stem cell-derived mesenchymal progenitor (iPSC-MP) cells were cultured on Ti model disks for 2 weeks in osteogenic conditions. Samples were then treated using four different decellularization methods to wash off the cells and expose the matrix. The functionalized disks were finally sterilized and seeded with fresh human iPSC-MP cells to study the effect of stem cell-mediated surface functionalization on cell behavior. The results show that different decellularization methods produce diverse surface modifications, and that these modifications promote proliferation of human iPSC-MP cells, affect the expression of genes involved in development and differentiation, and stimulate the release of alkaline phosphatase. Cell-mediated functionalization represents an attractive strategy to modify the surface of prosthetic implants with cues of biological relevance, and opens unprecedented possibilities for development of new devices with enhanced therapeutic potential.

Place, publisher, year, edition, pages
2017. Vol. 28, no 9, article id 133
National Category
Materials Engineering
Identifiers
URN: urn:nbn:se:uu:diva-333953DOI: 10.1007/s10856-017-5944-1ISI: 000407947900004PubMedID: 28744615OAI: oai:DiVA.org:uu-333953DiVA, id: diva2:1165515
Available from: 2017-12-13 Created: 2017-12-13 Last updated: 2018-02-08Bibliographically approved

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Pujari-Palmer, MichaelXia, WeiEngqvist, Håkan

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