uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Host cell recruitment patterns by bone morphogenetic protein-2 releasing hyaluronic acid hydrogels in a mouse subcutaneous environment
Univ Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy..
Univ Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy.;Alexandria Univ, Conservat Dent Dept, Endodont, Fac Dent, Alexandria, Egypt..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.ORCID iD: 0000-0001-6947-9601
Show others and affiliations
2017 (English)In: Regenerative Medicine, ISSN 1746-0751, E-ISSN 1746-076X, Vol. 12, no 5, 525-539 p.Article in journal (Refereed) Published
Abstract [en]

Aim: This study aimed to identify host cell recruitment patterns in a mouse model in response to rhBMP-2 releasing hyaluronic acid hydrogels and influence of added nano-hydroxyapatite particles on rhBMP-2 release and pattern of bone formation. Materials & methods: Implanted gels were retrieved after implantation and cells were enzymatically dissociated for flow cytometric analysis. Percentages of macrophages, progenitor endothelial cells and putative mesenchymal stem cells were measured. Implants were evaluated for BMP-2 release by ELISA and by histology to monitor tissue formation. Results & conclusion: Hyaluronic acid+BMP-2 gels influenced the inflammatory response in the bone healing microenvironment. Host-derived putative mesenchymal stem cells were major contributors. Addition of hydroxyapatite nanoparticles modified the release pattern of rhBMP-2, resulting in enhanced bone formation.

Place, publisher, year, edition, pages
FUTURE MEDICINE LTD , 2017. Vol. 12, no 5, 525-539 p.
Keyword [en]
BMP-2, cell recruitment, controlled release, endothelial progenitor cells, hyaluronic acid hydrogel, macrophages, putative mesenchymal stem cells
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-335685DOI: 10.2217/rme-2017-0023ISI: 000409196400006PubMedID: 28770657OAI: oai:DiVA.org:uu-335685DiVA: diva2:1167906
Funder
EU, FP7, Seventh Framework Programme, FP7-NMP-214402
Available from: 2017-12-19 Created: 2017-12-19 Last updated: 2017-12-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Varghese, Oommen P.Hilborn, Jöns

Search in DiVA

By author/editor
Varghese, Oommen P.Hilborn, Jöns
By organisation
Polymer Chemistry
In the same journal
Regenerative Medicine
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 12 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf